Dubina E V, Mukhina Zh M, Kharitonov E M, Shilovskiy V N, Kharchenko E S, Esaulova L V, Korkina N N, Maximenko E P, Nikitina I B
Genetika. 2015 Aug;51(8):881-6.
Based on modern technologies of molecular DNA-markers, blast disease-resistance genes (Pi-ta, Pi-b, Pi-1, Pi-2, and Pi-33) were introgressed and pyramided into domestic rice varieties to give them long-term disease resistance. For that purpose, this case study uses SSR-markers closely linked to these genes, as well as intragenic markers of genes Pi-ta and Pi-b. Multiplex PCR systems were created for simultaneous identification of two resistance genes in the hybrid progeny for the following combinations: Pi-1 + Pi-2, Pi-ta + Pi-b, Pi-ta + Pi-33.
基于现代分子DNA标记技术，将稻瘟病抗性基因（Pi-ta、Pi-b、Pi-1、Pi-2和Pi-33）导入并聚合到国内水稻品种中，使其具有长期抗病性。为此，本案例研究使用了与这些基因紧密连锁的SSR标记以及基因Pi-ta和Pi-b的基因内标记。构建了多重PCR体系，用于同时鉴定杂交后代中以下组合的两个抗性基因：Pi-1 + Pi-2、Pi-ta + Pi-b、Pi-ta + Pi-33。
