Wang Ruisen, Fang Nengyan, Guan Changhong, He Wanwan, Bao Yongmei, Zhang Hongsheng
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, College of Agriculture, Nanjing Agricultural University, Nanjing, China.
PLoS One. 2016 Dec 30;11(12):e0169417. doi: 10.1371/journal.pone.0169417. eCollection 2016.
Rice blast is a destructive disease caused by Magnaporthe oryzae, and it has a large impact on rice production worldwide. Compared with leaf blast resistance, our understanding of panicle blast resistance is limited. The japonica landrace Jiangnanwan from Taihu Lake region in China shows highly resistance to panicle and leaf blast. In this study, three generations (F2:5, F2:6, F2:7) consisting of 221 RILs (recombination inbreeding lines), developed from the cross of Jiangnanwan and Suyunuo, a susceptible-blast japonica variety, were evaluated for panicle blast resistance in the fields and leaf blast resistance in greenhouse in Nanjing in 2013, 2014 and 2015. A blast resistance gene Pi-jnw1 referring to panicle blast resistance and leaf blast resistance was identified in the three generations and located in the region of RM27273 and RM27381 in chromosome 11. The RIL18 line harboring Pi-jnw1 was selected to be backcrossed with Suyunuo to develop BC2F2 populations. According to the genotyping of 1,150 BC2F2 individuals and panicle blast and leaf blast resistance evaluation of 47 recombinants between RM27150 and RM27381, Pi-jnw1 was finally mapped to the 282 kb region between markers W28 and BS39. This study revealed that Jiangnanwan harboring a panicle blast and leaf blast resistance gene Pi-jnw1 could be a genetic source for breeding new rice cultivars with panicle blast resistance.
稻瘟病是由稻瘟病菌引起的一种毁灭性病害，对全球水稻生产有重大影响。与叶瘟抗性相比，我们对穗瘟抗性的了解有限。中国太湖地区的粳稻地方品种江南晚对穗瘟和叶瘟表现出高抗性。在本研究中，由江南晚与感病粳稻品种苏御糯杂交产生的包含221个重组自交系（RILs）的三个世代（F2:5、F2:6、F2:7），于2013年、2014年和2015年在南京进行了田间穗瘟抗性和温室叶瘟抗性评价。在这三个世代中鉴定出一个与穗瘟抗性和叶瘟抗性相关的抗瘟基因Pi-jnw1，并将其定位在第11染色体上的RM27273和RM27381区域。选择携带Pi-jnw1的RIL18株系与苏御糯回交，构建BC2F2群体。根据对1150个BC2F2个体的基因分型以及RM27150和RM27381之间47个重组体的穗瘟和叶瘟抗性评价，最终将Pi-jnw1定位到标记W28和BS39之间的282 kb区域。本研究表明，携带穗瘟和叶瘟抗性基因Pi-jnw1的江南晚可作为培育抗穗瘟水稻新品种的遗传资源。
