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Antibody anarchy: A call to order.

作者信息

Baker Monya

机构信息

Nature in San Francisco, California.

出版信息

Nature. 2015 Nov 26;527(7579):545-51. doi: 10.1038/527545a.

DOI:10.1038/527545a

PMID:26607547

Abstract

摘要

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Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.

Nat Methods. 2015 Aug;12(8):725-31. doi: 10.1038/nmeth.3472. Epub 2015 Jun 29.

Protein binder woes.

Nat Methods. 2015 May;12(5):373. doi: 10.1038/nmeth.3397.

Reproducibility: Standardize antibodies used in research.

Nature. 2015 Feb 5;518(7537):27-9. doi: 10.1038/518027a.

On the reproducibility of science: unique identification of research resources in the biomedical literature.

PeerJ. 2013 Sep 5;1:e148. doi: 10.7717/peerj.148. eCollection 2013.

Antibody validation.

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MUC2 mucin is a major carrier of the cancer-associated sialyl-Tn antigen in intestinal metaplasia and gastric carcinomas.

Glycobiology. 2010 Feb;20(2):199-206. doi: 10.1093/glycob/cwp161. Epub 2009 Oct 8.

Antibody array analysis with label-based detection and resolution of protein size.

Mol Cell Proteomics. 2009 Feb;8(2):245-57. doi: 10.1074/mcp.M800171-MCP200. Epub 2008 Sep 16.

Magic peptides, magic antibodies: guidelines for appropriate controls for immunohistochemistry.

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Antibody anarchy: A call to order.

作者信息

Baker Monya

机构信息

Nature in San Francisco, California.

出版信息

Nature. 2015 Nov 26;527(7579):545-51. doi: 10.1038/527545a.

DOI:10.1038/527545a

PMID:26607547

Abstract

摘要

相似文献

Antibody anarchy: A call to order.

Nature. 2015 Nov 26;527(7579):545-51. doi: 10.1038/527545a.

Reproducibility crisis: Blame it on the antibodies.

Nature. 2015 May 21;521(7552):274-6. doi: 10.1038/521274a.

Antibodies: The generation game.

Nature. 2007 Jun 7;447(7145):741-4. doi: 10.1038/447741a.

Policy on characterization of antibodies used in immunochemical methods of analysis for mycotoxins and phycotoxins.

J AOAC Int. 2003 Jul-Aug;86(4):868-71.

When antibodies mislead: the quest for validation.

Nature. 2020 Sep;585(7824):313-314. doi: 10.1038/d41586-020-02549-1.

Although it's painful: The importance of stringent antibody validation.

PLoS Pathog. 2018 Jan 4;14(1):e1006701. doi: 10.1371/journal.ppat.1006701. eCollection 2018 Jan.

The antibody/antiserum as an analytical reagent in quantitative immunoassays.

Scand J Clin Lab Invest Suppl. 1991;205:79-85. doi: 10.3109/00365519109104605.

Unusual results from immunoassays and the role of the clinical endocrinologist.

Clin Endocrinol (Oxf). 2006 Mar;64(3):234-44. doi: 10.1111/j.1365-2265.2006.02439.x.

Non-specific antibody interactions.

Biotechniques. 2012 May;52(5):305-6. doi: 10.2144/000113849.

Antibody Colocalization Microarray for Cross-Reactivity-Free Multiplexed Protein Analysis.

Methods Mol Biol. 2017;1619:239-261. doi: 10.1007/978-1-4939-7057-5_19.

引用本文的文献

Open-source antibodies as a path to enhanced research reproducibility and transparency.

N Biotechnol. 2025 Jul 25;87:121-129. doi: 10.1016/j.nbt.2025.04.004. Epub 2025 Apr 17.

Development of Recombinant Antibodies and Its Application in Immunomagnetic Separation-Based Rapid Detection of in Aquatic Environments.

J Microbiol Biotechnol. 2024 Nov 28;34(11):2266-2278. doi: 10.4014/jmb.2405.05003. Epub 2024 Oct 1.

Multiplex Determination of Glycan Profiles on Urinary Prostate-Specific Antigen by Quartz-Crystal Microbalance Combined with Surface-Enhanced Raman Scattering.

ACS Sens. 2024 Sep 27;9(9):4811-4821. doi: 10.1021/acssensors.4c01252. Epub 2024 Aug 30.

Antibody characterization is critical to enhance reproducibility in biomedical research.

Elife. 2024 Aug 14;13:e100211. doi: 10.7554/eLife.100211.

Improving the integrity and reproducibility of research that uses antibodies: a technical, data sharing, behavioral and policy challenge.

MAbs. 2024 Jan-Dec;16(1):2323706. doi: 10.1080/19420862.2024.2323706. Epub 2024 Mar 6.

Developing a Toolbox of Antibodies Validated for Array Tomography-Based Imaging of Brain Synapses.

eNeuro. 2023 Dec 22;10(12). doi: 10.1523/ENEURO.0290-23.2023. Print 2023 Dec.

YCharOS open antibody characterisation data: Lessons learned and progress made.

F1000Res. 2023 Oct 16;12:1344. doi: 10.12688/f1000research.141719.1. eCollection 2023.

Bovine Serum Albumin Molecularly Imprinted Electrochemical Sensors Modified by Carboxylated Multi-Walled Carbon Nanotubes/CaAlg Hydrogels.

Gels. 2023 Aug 20;9(8):673. doi: 10.3390/gels9080673.

Developing a Toolbox of Antibodies Validated for Array Tomography-Based Imaging of Brain Synapses.

bioRxiv. 2023 Nov 8:2023.06.28.546920. doi: 10.1101/2023.06.28.546920.

Single-Cell Proteomics with Spatial Attributes: Tools and Techniques.

ACS Omega. 2023 May 9;8(20):17499-17510. doi: 10.1021/acsomega.3c00795. eCollection 2023 May 23.

本文引用的文献

How to avoid pitfalls in antibody use.

F1000Res. 2015 Sep 7;4:691. doi: 10.12688/f1000research.6894.1. eCollection 2015.

Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.

Nat Methods. 2015 Aug;12(8):725-31. doi: 10.1038/nmeth.3472. Epub 2015 Jun 29.

Protein binder woes.

Nat Methods. 2015 May;12(5):373. doi: 10.1038/nmeth.3397.

Reproducibility: Standardize antibodies used in research.

Nature. 2015 Feb 5;518(7537):27-9. doi: 10.1038/518027a.

On the reproducibility of science: unique identification of research resources in the biomedical literature.

PeerJ. 2013 Sep 5;1:e148. doi: 10.7717/peerj.148. eCollection 2013.

Antibody validation.

Biotechniques. 2010 Mar;48(3):197-209. doi: 10.2144/000113382.

MUC2 mucin is a major carrier of the cancer-associated sialyl-Tn antigen in intestinal metaplasia and gastric carcinomas.

Glycobiology. 2010 Feb;20(2):199-206. doi: 10.1093/glycob/cwp161. Epub 2009 Oct 8.

Antibody array analysis with label-based detection and resolution of protein size.

Mol Cell Proteomics. 2009 Feb;8(2):245-57. doi: 10.1074/mcp.M800171-MCP200. Epub 2008 Sep 16.

Magic peptides, magic antibodies: guidelines for appropriate controls for immunohistochemistry.

J Comp Neurol. 2003 Oct 13;465(2):161-3. doi: 10.1002/cne.10858.