Department of Biochemistry, Emory University School of Medicine, Atlanta, United States.
Department of Respiratory Sciences, University of Leicester, Leicester, United Kingdom.
Elife. 2024 Aug 14;13:e100211. doi: 10.7554/eLife.100211.
Antibodies are used in many areas of biomedical and clinical research, but many of these antibodies have not been adequately characterized, which casts doubt on the results reported in many scientific papers. This problem is compounded by a lack of suitable control experiments in many studies. In this article we review the history of the 'antibody characterization crisis', and we document efforts and initiatives to address the problem, notably for antibodies that target human proteins. We also present recommendations for a range of stakeholders - researchers, universities, journals, antibody vendors and repositories, scientific societies and funders - to increase the reproducibility of studies that rely on antibodies.
抗体在生物医学和临床研究的许多领域都有应用，但其中许多抗体的特性尚未得到充分表征，这使得许多科学论文中报告的结果受到质疑。许多研究中缺乏合适的对照实验，使这个问题更加严重。在本文中，我们回顾了“抗体特性危机”的历史，并记录了为解决这一问题所做的努力和倡议，特别是针对针对人类蛋白的抗体。我们还为一系列利益相关者（研究人员、大学、期刊、抗体供应商和储存库、科学协会和资助者）提供了建议，以提高依赖抗体的研究的可重复性。
