Campero C M, Ladds P W, Hoffmann D, De'ath G
Graduate School of Tropical Veterinary Science, James Cook University of North Queensland, Townsville.
Aust Vet J. 1989 May;66(5):137-41. doi: 10.1111/j.1751-0813.1989.tb09779.x.
The peroxidase-antiperoxidase (PAP) technique was used to identify cytoplasmic immunoglobulins in the accessory sex glands of 15 normal bulls and 13 bulls with inflammation of the ASG. Immunoglobulin containing cells (ICC) of the types IgA, IgM, total IgG, IgG1 and IgG2 were measured and their percentages expressed. In accessory sex glands from normal bulls, IgA containing cells were the most frequent in prostate and bulbourethral glands (86.7% and 86.1%, respectively of all ICC present) whereas in the ampulla, IgG containing cells comprised 78.6% of the ICC. IgG1 and IgG2 containing cells were present in all the accessory sex glands in approximately equal numbers. Frequencies of IgM containing cells in the ampulla, prostate and bulbourethral glands were 6.3%, 4.0% and 3.7%, respectively. Although all isotypes of ICC were present in the seminal vesicle, the very low number precluded accurate quantification. In inflamed ampulla, seminal vesicle, bulbourethral gland and colliculus seminalis, IgG containing cells were the most frequent ICC with values of 66.2%, 83.0%, 69.0% and 53.5%, respectively; IgA containing cells were the second in prevalence with values of 21.5%, 10.3% 19.3% and 40.5%, respectively. The contribution of ICC to the locally protective immunoglobulins in accessory sex gland secretions is discussed.
采用过氧化物酶-抗过氧化物酶（PAP）技术，对15头正常公牛和13头患有附属生殖腺炎的公牛的附属生殖腺中的细胞质免疫球蛋白进行鉴定。测定了IgA、IgM、总IgG、IgG1和IgG2类型的含免疫球蛋白细胞（ICC），并给出其百分比。在正常公牛的附属生殖腺中，含IgA细胞在前列腺和尿道球腺中最为常见（分别占所有存在的ICC的86.7%和86.1%），而在壶腹中，含IgG细胞占ICC的78.6%。含IgG1和IgG2的细胞在所有附属生殖腺中的数量大致相等。壶腹、前列腺和尿道球腺中含IgM细胞的频率分别为6.3%、4.0%和3.7%。虽然精囊中有所有ICC同种型，但数量极少，无法进行准确量化。在发炎的壶腹、精囊、尿道球腺和精阜中，含IgG细胞是最常见的ICC，其值分别为66.2%、83.0%、69.0%和53.5%；含IgA细胞的患病率次之，分别为21.5%、10.3%、19.3%和40.5%。讨论了ICC对附属生殖腺分泌物中局部保护性免疫球蛋白的作用。
