Foster R A, Ladds P W, Husband A J, Hoffmann D
Graduate School of Tropical Veterinary Science, James Cook University of North Queensland, Townsville.
Aust Vet J. 1988 Feb;65(2):37-40. doi: 10.1111/j.1751-0813.1988.tb07348.x.
Thirteen rams with serological evidence of Brucella ovis exposure (CFT of 1:8 or greater), but with no or only mild epididymitis, were selected from a ram flock. Serum, semen, preputial washings and fluids from the accessory sex glands (ASGF) and testis and epididymis (TEF) were examined and immunoglobulin (Ig) concentrations estimated. Genital tissues were examined histologically and the percentages of class specific immunoglobulin containing cells (ICC) determined. Eleven of these rams had histological evidence of active inflammation consistent with B. ovis infection; the organisam was cultured from the semen of 7. IgA concentration was high in semen (mean +/- standard deviation of 5.03 +/- 1.78 mg/ml) and ASGF (9.18 +/- 7.28 mg/ml). These levels were much higher than those recorded in noninfected rams. IgA concentration was low in serum (0.78 +/- 0.55 mg/ml) and TEF (0.59 +/- 0.78 mg/ml). The concentrations of IgG1, IgG2 and IgM were low in all genital fluids sampled and not significantly different from those recorded in noninfected rams. This indicated that infection with B. ovis results in a pronounced IgA response in secretions, mostly from the accessory sex glands. Examinations of ICC, however, revealed that the plasma cell infiltrates of the epididymis, vas deferens, ampulla and seminal vesicle were predominantly IgG-containing (92.4, 97.2, 79.4 and 91.9% respectively). Fewer IgM-containing cells were scattered throughout these tissues, constituting 3.9, 6.3, 0.3 and 6.5% of all ICC, respectively. IgA-containing cells were most frequently seen in the ampulla (9.6% of ICC) where they were located directly beneath the epithelium, suggesting the ampulla as the most prominant location for the local production of IgA.(ABSTRACT TRUNCATED AT 250 WORDS)
从一群公羊中挑选出13只血清学检测显示有绵羊布鲁氏菌接触证据（试管凝集试验效价为1:8或更高）但无附睾炎或仅有轻度附睾炎的公羊。检测了血清、精液、包皮冲洗液以及附属性腺（ASGF）、睾丸和附睾（TEF）的液体，并估算了免疫球蛋白（Ig）浓度。对生殖组织进行了组织学检查，并确定了各类特异性含免疫球蛋白细胞（ICC）的百分比。其中11只公羊有与绵羊布鲁氏菌感染相符的活动性炎症组织学证据；从7只公羊的精液中培养出了该病原体。精液（平均±标准差为5.03±1.78毫克/毫升）和附属性腺（9.18±7.28毫克/毫升）中的IgA浓度较高。这些水平远高于未感染公羊的记录水平。血清（0.78±0.55毫克/毫升）和睾丸附睾液（0.59±0.78毫克/毫升）中的IgA浓度较低。所有采集的生殖液中IgG1、IgG2和IgM的浓度较低，与未感染公羊的记录水平无显著差异。这表明绵羊布鲁氏菌感染导致分泌物中出现明显的IgA反应，主要来自附属性腺。然而，对ICC的检查显示，附睾、输精管、壶腹和精囊的浆细胞浸润主要含IgG（分别为92.4%、97.2%、79.4%和91.9%）。较少的含IgM细胞散布于这些组织中，分别占所有ICC的3.9%、6.3%、0.3%和6.5%。含IgA细胞最常见于壶腹（占ICC的9.6%），它们直接位于上皮下方，表明壶腹是局部产生IgA的最主要部位。（摘要截短于250词）
