Marques Cláudia N H, Davies David G, Sauer Karin
Department of Biological Sciences, Binghamton University, Binghamton, NY 13902, USA.
Binghamton Biofilm Research Center (BBRC), Binghamton University, Binghamton, NY 13902, USA.
Pharmaceuticals (Basel). 2015 Nov 25;8(4):816-35. doi: 10.3390/ph8040816.
Biofilms are complex communities of microorganisms in organized structures attached to surfaces. Importantly, biofilms are a major cause of bacterial infections in humans, and remain one of the most significant challenges to modern medical practice. Unfortunately, conventional therapies have shown to be inadequate in the treatment of most chronic biofilm infections based on the extraordinary innate tolerance of biofilms to antibiotics. Antagonists of quorum sensing signaling molecules have been used as means to control biofilms. QS and other cell-cell communication molecules are able to revert biofilm tolerance, prevent biofilm formation and disrupt fully developed biofilms, albeit with restricted effectiveness. Recently however, it has been demonstrated that Pseudomonas aeruginosa produces a small messenger molecule cis-2-decenoic acid (cis-DA) that shows significant promise as an effective adjunctive to antimicrobial treatment of biofilms. This molecule is responsible for induction of the native biofilm dispersion response in a range of Gram-negative and Gram-positive bacteria and in yeast, and has been shown to reverse persistence, increase microbial metabolic activity and significantly enhance the cidal effects of conventional antimicrobial agents. In this manuscript, the use of cis-2-decenoic acid as a novel agent for biofilm control is discussed. Stimulating the biofilm dispersion response as a novel antimicrobial strategy holds significant promise for enhanced treatment of infections and in the prevention of biofilm formation.
生物膜是附着于表面的有组织结构中的微生物复杂群落。重要的是，生物膜是人类细菌感染的主要原因，并且仍然是现代医学实践面临的最重大挑战之一。不幸的是，基于生物膜对抗生素具有非凡的固有耐受性，传统疗法在治疗大多数慢性生物膜感染方面已显示出不足。群体感应信号分子拮抗剂已被用作控制生物膜的手段。群体感应和其他细胞间通讯分子能够逆转生物膜耐受性、防止生物膜形成并破坏完全形成的生物膜，尽管效果有限。然而最近，已证明铜绿假单胞菌产生一种小信使分子顺式-2-癸烯酸（cis-DA），它作为生物膜抗菌治疗的有效辅助剂显示出巨大潜力。该分子负责在一系列革兰氏阴性菌、革兰氏阳性菌和酵母中诱导天然生物膜分散反应，并且已证明它能逆转持续性、增加微生物代谢活性并显著增强传统抗菌剂的杀菌效果。在本手稿中，讨论了使用顺式-2-癸烯酸作为控制生物膜的新型药物。刺激生物膜分散反应作为一种新型抗菌策略在增强感染治疗和预防生物膜形成方面具有巨大潜力。
