Wakasa Yuhya, Takaiwa Fumio
Functional Transgenic Crops Research Unit, Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan.
Methods Mol Biol. 2016;1385:223-47. doi: 10.1007/978-1-4939-3289-4_16.
Rice seeds are an ideal production platform for high-value recombinant proteins in terms of economy, scalability, safety, and stability. Strategies for the expression of large amounts of recombinant proteins in rice seeds have been established in the past decade and transgenic rice seeds that accumulate recombinant products such as bioactive peptides and proteins, which promote the health and quality of life of humans, have been generated in many laboratories worldwide. One of the most important advantages is the potential for direct oral delivery of transgenic rice seeds without the need for recombinant protein purification (downstream processing), which has been attributed to the high expression levels of recombinant products. Transgenic rice will be beneficial as a delivery system for pharmaceuticals and nutraceuticals in the future. This chapter introduces the strategy for producing recombinant protein in the edible part (endosperm) of the rice grain and describes methods for the analysis of transgenic rice seeds in detail.
就经济性、可扩展性、安全性和稳定性而言，水稻种子是生产高价值重组蛋白的理想平台。在过去十年中已确立了在水稻种子中大量表达重组蛋白的策略，并且全世界许多实验室都已培育出积累重组产物（如生物活性肽和蛋白质）的转基因水稻种子，这些产物可促进人类健康和提高生活质量。其中一个最重要的优势是转基因水稻种子有可能直接口服，而无需进行重组蛋白纯化（下游加工），这归因于重组产物的高表达水平。转基因水稻未来将作为药物和营养保健品的递送系统发挥作用。本章介绍了在水稻籽粒可食用部分（胚乳）生产重组蛋白的策略，并详细描述了转基因水稻种子的分析方法。
