Hayashi Mitsuha, Yanaba Koichi, Umezawa Yoshinori, Yoshihara Yuki, Kikuchi Sota, Ishiuji Yozo, Saeki Hidehisa, Nakagawa Hidemi
Department of Dermatology, The Jikei University School of Medicine, Tokyo, Japan.
Department of Dermatology, The Jikei University School of Medicine, Tokyo, Japan.
J Dermatol Sci. 2016 Feb;81(2):93-100. doi: 10.1016/j.jdermsci.2015.11.003. Epub 2015 Nov 14.
Interleukin (IL)-10-producing regulatory B cells (B10 cells) have been shown to ameliorate psoriasis in mice. Human B10 progenitor cells are characterized as CD19(+)CD24(hi)CD38(hi) B cells that exert their regulatory functions via the production of IL-10. However, the role of B10 cells in the pathogenesis of psoriasis remains unclear.
We examined B10 cells in patients with psoriasis and healthy controls.
Peripheral blood mononuclear cells were isolated from psoriasis patients without a history of receiving any immunosuppressants during the 6-month period before enrollment in the study. Using flow cytometry, we determined the frequencies of blood B cell subsets, B10 progenitor cells, and B10 cells for 31 patients with psoriasis and 26 healthy controls.
Both psoriasis patients and healthy controls showed similar frequencies of total B cells, IgD(+)CD27(-) naïve B cells, and IgD(-)CD27(+) memory B cells. However, the frequency of CD19(+)CD24(hi)CD38(hi) B10 progenitor cells was significantly higher in patients with psoriasis than in the healthy controls. In contrast, the frequency of B10 cells in patients with psoriasis was significantly lower than that in healthy controls. Furthermore, treatment with immunosuppressants resulted in a decrease in B10 progenitor cells and an increase in B10 cells.
B10 progenitor cells were increased, while IL-10-producing regulatory B10 cells were decreased in patients with psoriasis, suggesting that B10 cells may be functionally impaired in patients with psoriasis.
已证实白细胞介素（IL）-10产生性调节B细胞（B10细胞）可改善小鼠银屑病。人B10祖细胞的特征为CD19(+)CD24(hi)CD38(hi) B细胞，其通过产生IL-10发挥调节功能。然而，B10细胞在银屑病发病机制中的作用仍不清楚。
我们检测了银屑病患者和健康对照者体内的B10细胞。
从在研究入组前6个月内无任何免疫抑制剂使用史的银屑病患者中分离外周血单个核细胞。使用流式细胞术，我们测定了31例银屑病患者和26例健康对照者血液B细胞亚群、B10祖细胞和B10细胞的频率。
银屑病患者和健康对照者的总B细胞、IgD(+)CD27(-)幼稚B细胞和IgD(-)CD27(+)记忆B细胞频率相似。然而，银屑病患者中CD19(+)CD24(hi)CD38(hi) B10祖细胞的频率显著高于健康对照者。相反，银屑病患者中B10细胞的频率显著低于健康对照者。此外，免疫抑制剂治疗导致B10祖细胞减少，B10细胞增加。
银屑病患者中B10祖细胞增加，而产生IL-10的调节性B10细胞减少，提示银屑病患者的B10细胞可能存在功能受损。
