人牙龈边缘来源的干祖细胞的TlR表达谱
TlR expression profile of human gingival margin-derived stem progenitor cells.
作者信息
Fawzy-El-Sayed Karim, Mekhemar Mohamed, Adam-Klages Sabine, Kabelitz Dietrich, Dörfer Christof
机构信息
Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian Albrechts-Universität zu Kiel, Arnold-Heller-Str. 3, Haus 26, 24105 Kiel, Germany,
出版信息
Med Oral Patol Oral Cir Bucal. 2016 Jan 1;21(1):e30-8. doi: 10.4317/medoral.20593.
BACKGROUND
Gingival margin-derived stem/progenitor cells (G-MSCs) show remarkable periodontal regenerative potential in vivo. During regeneration, G-MSCs may interact with their inflammatory environment via toll-like-receptors (TLRs). The present study aimed to depict the G-MSCs TLRs expression profile.
MATERIAL AND METHODS
Cells were isolated from free gingival margins, STRO-1-immunomagnetically sorted and seeded to obtain single colony forming units (CFUs). G-MSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, CD146 and STRO-1 expression, and for multilineage differentiation potential. Following G-MSCs' incubation in basic or inflammatory medium (IL-1β, IFN-γ, IFN-α, TNF-α) a TLR expression profile was generated.
RESULTS
G-MSCs showed all stem/progenitor cells' characteristics. In basic medium G-MSCs expressed TLRs 1, 2, 3, 4, 5, 6, 7, and 10. The inflammatory medium significantly up-regulated TLRs 1, 2, 4, 5, 7 and 10 and diminished TLR 6 (p≤0.05, Wilcoxon-Signed-Ranks-Test).
CONCLUSIONS
The current study describes for the first time the distinctive TLRs expression profile of G-MSCs under uninflamed and inflamed conditions.
背景
牙龈缘来源的干/祖细胞（G-MSCs）在体内显示出显著的牙周再生潜力。在再生过程中，G-MSCs可能通过Toll样受体（TLRs）与其炎症环境相互作用。本研究旨在描绘G-MSCs的TLRs表达谱。
材料与方法
从游离牙龈缘分离细胞，通过STRO-1免疫磁珠分选并接种以获得单克隆集落形成单位（CFUs）。对G-MSCs进行CD14、CD34、CD45、CD73、CD90、CD105、CD146和STRO-1表达以及多向分化潜能的鉴定。在基础培养基或炎症培养基（IL-1β、IFN-γ、IFN-α、TNF-α）中培养G-MSCs后，生成TLR表达谱。
结果
G-MSCs表现出所有干/祖细胞的特征。在基础培养基中，G-MSCs表达TLRs 1、2、3、4、5、6、7和10。炎症培养基显著上调TLRs 1、2、4、5、7和10，并降低TLR 6（p≤0.05，Wilcoxon符号秩和检验）。
结论
本研究首次描述了G-MSCs在未发炎和发炎条件下独特的TLRs表达谱。
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