Clinic for Conservative Dentistry and Periodontology, School of Dental Medicine, Christian-Albrecht's University, Kiel, Germany.
Universitätsklinikum Schleswig Holstein, Institut für Immunologie, Kiel, Germany.
Cell Immunol. 2018 Apr;326:60-67. doi: 10.1016/j.cellimm.2017.01.007. Epub 2017 Jan 10.
During therapeutic application, mesenchymal stem cells (MSCs) may interact with their environment via their expressed toll-like-receptors (TLRs) leading to pro- or anti-inflammatory immune responses. The present study aimed to describe the gingival margin-derived stem/progenitor cells' (G-MSCs) TLR-induced immune regulatory response to specific TLR agonists. Gingival cells were obtained, immunomagnetically sorted via anti-STRO-1 antibodies and seeded out to achieve colony forming units (CFUs). G-MSCs were investigated for stem cell characteristics and TLR expression. Specific TLR agonists were applied and m-RNA expression of pro- and anti-inflammatory factors was analyzed via real-time polymerase chain reaction. G-MSCs showed all characteristics of stem/progenitor cells. All TLR agonists induced pro-inflammatory cytokines, except for the TLR3 agonist, which significantly promoted the anti-inflammatory response. (p⩽0.05, Wilcoxon-Signed-Ranks-Test). TLR-induced immunomodulation by G-MSCs could impact their therapeutic potential in vivo. Two distinctive pro-inflammatory and an anti-inflammatory TLR-induced phenotypes of G-MSCs become noticeable in this study.
在治疗应用中，间充质干细胞 (MSCs) 可能通过其表达的 toll 样受体 (TLRs) 与环境相互作用，导致促炎或抗炎免疫反应。本研究旨在描述牙龈缘来源的干细胞/祖细胞 (G-MSCs) 对特定 TLR 激动剂的 TLR 诱导的免疫调节反应。从牙龈中获取细胞，通过抗 STRO-1 抗体进行免疫磁珠分选，并接种以获得集落形成单位 (CFU)。研究 G-MSCs 的干细胞特性和 TLR 表达。应用特定的 TLR 激动剂，通过实时聚合酶链反应分析促炎和抗炎因子的 m-RNA 表达。G-MSCs 表现出所有的干细胞/祖细胞特征。除 TLR3 激动剂外，所有 TLR 激动剂均诱导促炎细胞因子，而 TLR3 激动剂则显著促进抗炎反应。(p ⩽ 0.05，Wilcoxon-Signed-Ranks-Test)。G-MSCs 的 TLR 诱导免疫调节可能会影响其在体内的治疗潜力。本研究中，G-MSCs 出现了两种明显的促炎和抗炎 TLR 诱导表型。
