Walsh Emily, Luo Jing, Naik Abhishek, Preteroti Thomas, Zhang Ning
Department of Plant Biology and Pathology, 201 Foran Hall, 59 Dudley Road, Rutgers University, New Brunswick, NJ 08901, USA.
Department of Plant Biology and Pathology, 201 Foran Hall, 59 Dudley Road, Rutgers University, New Brunswick, NJ 08901, USA; Department of Biochemistry and Microbiology, 76 Lipman Drive, Rutgers University, New Brunswick, NJ 08901, USA.
Fungal Biol. 2015 Dec;119(12):1216-1225. doi: 10.1016/j.funbio.2015.09.010. Epub 2015 Oct 13.
A new genus Barrenia is described based on multi-gene phylogenetic analyses and phenotypic and ecological characters. Isolated from roots of switchgrass and pitch pine in the acidic and oligotrophic New Jersey Pine Barrens in this study, Barrenia likely has a wide distribution because its internal transcribed spacer (ITS) sequence has high similarity with a number of GenBank sequences from various ecological studies. The majority of these matching samples were from roots of plants in acidic, nutrient-poor environments, as well as from managed sugarcane plantations. Phylogenetic analyses based on ITS, LSU, and RPB1 sequence data strongly support that Barrenia is a monophyletic clade in Helotiales, distinct from any known taxa. Barrenia is phylogenetically close to Acidomelania, Loramyces, Mollisia, and Phialocephala fortinii - Acephala applanata species complex (PAC), the dark septate endophytes. Barrenia can be distinguished from Loramyces and Mollisia by its association with living plant roots. Taxa in PAC also are root endophytes but they have complex phialid arrangements that appear to be lacking in Barrenia. Plant-fungal interaction experiments showed that Barrenia panicia and Acidomelania panicicola significantly promoted root hair growth in switchgrass. Results from this work will facilitate ecological and evolutionary studies on root-associated fungi.
基于多基因系统发育分析以及表型和生态特征，描述了一个新属——贫瘠菌属（Barrenia）。在本研究中，贫瘠菌属是从新泽西州贫瘠松林酸性且贫营养环境中的柳枝稷和北美油松根部分离得到的。由于其内部转录间隔区（ITS）序列与各种生态研究中的多个GenBank序列具有高度相似性，贫瘠菌属可能具有广泛的分布。这些匹配样本大多来自酸性、营养贫瘠环境中的植物根部，以及人工管理的甘蔗种植园。基于ITS、LSU和RPB1序列数据的系统发育分析有力地支持了贫瘠菌属是柔膜菌目（Helotiales）中的一个单系分支，与任何已知分类群都不同。在系统发育上，贫瘠菌属与酸黑霉属（Acidomelania）、洛拉霉菌属（Loramyces）、 Mollisia属以及暗隔内生真菌的福廷瓶霉 - 扁平无头瓶霉物种复合体（PAC）关系密切。贫瘠菌属可通过其与活植物根系的关联与洛拉霉菌属和Mollisia属区分开来。PAC中的分类群也是根内生菌，但它们具有复杂的瓶梗排列，而贫瘠菌属似乎没有这种排列。植物 - 真菌相互作用实验表明，潘氏贫瘠菌（Barrenia panicia）和恐慌酸黑霉（Acidomelania panicicola）显著促进了柳枝稷根毛的生长。这项工作的结果将有助于对与根相关真菌的生态和进化研究。
