Bi Xiaobao, Yang Renliang, Feng Xiaoyu, Rhodes Daniela, Liu Chuan-Fa
Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
Org Biomol Chem. 2016 Jan 21;14(3):835-9. doi: 10.1039/c5ob02323h. Epub 2015 Nov 30.
Using a genetically incorporated azidonorleucine for ubiquitin installation, we prepared multi-milligram quantities of H2AK119ub (ubH2A). With a native isopeptide linkage, the synthetic ubH2A was used to study the activity of deubiquitinases and crosstalk between H2A ubiquitination and H3K36 methylation in the context of chemically defined mononucleosomes.
我们利用基因编码的叠氮亮氨酸进行泛素化修饰，制备了多毫克量的H2AK119泛素化组蛋白（ubH2A）。该合成的ubH2A具有天然的异肽键，用于在化学定义的单核小体背景下研究去泛素化酶的活性以及H2A泛素化与H3K36甲基化之间的相互作用。
