CCR9和CCR7在重症肌无力患者的CD4 CD8胸腺细胞中过表达。
CCR9 AND CCR7 are overexpressed in CD4 CD8 thymocytes of myasthenia gravis patients.
作者信息
Li Qianru, Liu Pingping, Xuan Xiaoyan, Zhang Junfeng, Zhang Yun, Zhu Zhengkun, Gao Feng, Zhang Qingyong, Du Ying
机构信息
Department of Immunology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou 450001, Henan Province, PR China.
Department of Laboratory, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, PR China.
出版信息
Muscle Nerve. 2017 Jan;55(1):84-90. doi: 10.1002/mus.24999. Epub 2016 Oct 24.
INTRODUCTION
Chemokine CC motif receptors 9 and 7 (CCR9 and CCR7) play a major role in the migration of T-cell precursors to the thymus to initiate T thymopoiesis. However, their role in development of T-cells in myasthenia gravis (MG) patients has not been fully elucidated.
METHODS
Expression and distribution of CCR9 and CCR7 cells were detected by flow cytometry and immunofluorescence. Real-time polymerase chain reaction was used to check the adhesion molecules on CD4 CD8 double-negative (DN) thymocytes.
RESULTS
CCR9 and CCR7 expression by DN thymocytes increased in the MG thymus; the levels of CCR9, CCR7, interleukin-7R mRNA increased, and CXCR4 levels decreased compared with levels in the non-MG thymus. More CCR7 and CCR9 double-positive (DP) thymocytes were gathered near the subcapsular region in MG thymus.
CONCLUSIONS
Enhanced expression of CCR9 and CCR7 may complicate the differentiation of DP thymocytes from the DN stage in MG thymus. Muscle Nerve, 2016 Muscle Nerve 55: 84-90, 2017.
引言
趋化因子CC基序受体9和7（CCR9和CCR7）在T细胞前体迁移至胸腺以启动T细胞胸腺生成过程中起主要作用。然而，它们在重症肌无力（MG）患者T细胞发育中的作用尚未完全阐明。
方法
采用流式细胞术和免疫荧光法检测CCR9和CCR7细胞的表达及分布。运用实时聚合酶链反应检测CD4 CD8双阴性（DN）胸腺细胞上的黏附分子。
结果
MG胸腺中DN胸腺细胞的CCR9和CCR7表达增加；与非MG胸腺相比，CCR9、CCR7、白细胞介素-7R mRNA水平升高，而CXCR4水平降低。MG胸腺中更多CCR7和CCR9双阳性（DP）胸腺细胞聚集在被膜下区域附近。
结论
CCR9和CCR7表达增强可能使MG胸腺中DP胸腺细胞从DN阶段的分化复杂化。《肌肉与神经》，2016年 《肌肉与神经》55: 84 - 90, 2017年。
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