do Carmo Guilherme M, Crivellenti Leandro Z, Bottari Nathieli B, Machado Gustavo, Borin-Crivellenti Sofia, Moresco Rafael N, Duarte Thiago, Duarte Marta, Tinucci-Costa Mirela, Morsch Vera M, Schetinger Maria Rosa C, Stefani Lenita M, Da Silva Aleksandro S
Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Department of Veterinary Clinic and Surgery, Universidade Estadual Paulista "Júlio de Mesquita Filho", Jaboticabal, SP, Brazil; Department of Veterinary Clinic and Surgery, Universidade de Franca, Franca, SP, Brazil.
Comp Immunol Microbiol Infect Dis. 2015 Dec;43:16-21. doi: 10.1016/j.cimid.2015.09.005. Epub 2015 Oct 9.
The aim of this study was to evaluate the role of butyrylcholinesterase (BChE) as a marker of inflammation and liver injury in the acute and subclinical phases of canine ehrlichiosis. Forty-two serum samples of dogs naturally infected with Ehrlichia canis were used, of which 24 were from animals with the acute phase of the disease and 18 with subclinical disease. In addition, sera from 17 healthy dogs were used as negative controls. The hematocrit, BChE activity, hepatic injury (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)), nitric oxide, and cytokines levels were evaluated. The BChE activity was significantly elevated (P<0.05) in dogs with the acute phase of the disease when compared to healthy animals. However, there was a reduction on BChE activity on dogs with subclinical disease compared to the other two groups. AST and ALT levels were significantly higher (P<0.05) in the acute phase, as well as the inflammatory mediators (NOx, TNF-α, INF-γ, IL-4, IL-6) when compared to the control group. On the other hand, IL-10 levels were lower in the acute phase. Based on these results, we are able to conclude that the acute infection caused by E. canis in dogs leads to an increase on seric BChE activity and some inflammatory mediators. Therefore, this enzyme might be used as a marker of acute inflammatory response in dogs naturally infected by this bacterium.
本研究的目的是评估丁酰胆碱酯酶（BChE）作为犬埃立克体病急性和亚临床阶段炎症及肝损伤标志物的作用。使用了42份自然感染犬埃立克体的犬血清样本，其中24份来自处于疾病急性期的动物，18份来自亚临床疾病动物。此外，将17只健康犬的血清用作阴性对照。评估了血细胞比容、BChE活性、肝损伤（丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST））、一氧化氮和细胞因子水平。与健康动物相比，处于疾病急性期的犬BChE活性显著升高（P<0.05）。然而，与其他两组相比，亚临床疾病犬的BChE活性有所降低。与对照组相比，急性期的AST和ALT水平以及炎症介质（NOx、TNF-α、INF-γ、IL-4、IL-6）显著更高（P<0.05）。另一方面，急性期的IL-10水平较低。基于这些结果，我们能够得出结论，犬感染犬埃立克体引起的急性感染会导致血清BChE活性和一些炎症介质增加。因此，这种酶可能用作自然感染该细菌的犬急性炎症反应的标志物。
