Stanilov Iskren, Gospodinova Krasimira, Petrov Vladimir, Miteva Lyuba, Tsachev Ilia, Stanilova Spaska
Department of Hygiene, Epidemiology, Microbiology, Parasitology and Infectious Diseases, Medical Faculty, Trakia University, 6000 Stara Zagora, Bulgaria.
Department of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria.
Microorganisms. 2024 Dec 6;12(12):2516. doi: 10.3390/microorganisms12122516.
Infection of dogs with the tick-borne rickettsiae and provokes an immune response mediating the pathology and bacterial resistance. IL-10 is the main anti-inflammatory cytokine and plays a multifaceted role in host protection. The study aimed to investigate circulating IL-10 in 32 dogs naturally infected with and , identified by PCR positivity, and 33 PCR-negative animals which tested positive for antibodies against these pathogens, as well as 22 healthy animals. The highest quantity of IL-10, measured by ELISA, was observed among dogs positive simultaneously for anti- and anti-A. IgG antibodies, followed by dogs positive for anti- only. The concentration of IL-10 in PCR-positive dogs was almost three and a half times higher than that measured in the control group (77.09 ± 23.61 pg./mL vs. 21.55 ± 4.61 pg./mL; = 0.0015) and five times higher than the concentration of interleukin in PCR-negative animals (77.09 ± 23.61 pg./mL vs. 14.86 ± 3.01 pg./mL; = 0.000016). The highest level of IL-10 was observed in PCR-positive dogs with mixed infection (120.54 ± 44.18), followed by the level in PCR-positive dogs for only (78.81 ± 16.92). The lowest level of IL-10 was observed in PCR-positive dogs for only (56.32 ± 12.68). We may suggest that infection with and stimulates the IL-10 production in dogs, which may facilitate specific antibody responses.
犬感染蜱传立克次体后会引发免疫反应，介导病理变化和细菌耐药性。白细胞介素-10是主要的抗炎细胞因子，在宿主保护中发挥多方面作用。本研究旨在调查32只经聚合酶链反应（PCR）检测呈阳性、自然感染了[病原体名称1]和[病原体名称2]的犬，33只针对这些病原体抗体检测呈阳性但PCR检测阴性的动物，以及22只健康动物体内循环白细胞介素-10的情况。通过酶联免疫吸附测定（ELISA）法测得，同时针对[病原体名称1]和[病原体名称2] IgG抗体呈阳性的犬体内白细胞介素-10含量最高，其次是仅针对[病原体名称1]呈阳性的犬。PCR检测呈阳性的犬体内白细胞介素-10浓度几乎是对照组的三倍半（77.09 ± 23.61 pg./mL对21.55 ± 4.61 pg./mL；P = 0.0015），是PCR检测阴性动物体内白细胞介素浓度的五倍（77.09 ± 23.61 pg./mL对14.86 ± 3.01 pg./mL；P = 0.000016）。混合感染的PCR检测呈阳性的犬体内白细胞介素-10水平最高（120.54 ± 44.18），其次是仅感染[病原体名称1]的PCR检测呈阳性的犬（78.81 ± 16.92）。仅感染[病原体名称2]的PCR检测呈阳性的犬体内白细胞介素-10水平最低（56.32 ± 12.68）。我们可以推测，感染[病原体名称1]和[病原体名称2]会刺激犬体内白细胞介素-10的产生，这可能有助于特异性抗体反应。
