Kobus Thiele, Vykhodtseva Natalia, Pilatou Magdalini, Zhang Yongzhi, McDannold Nathan
Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Department of Radiology and Nuclear Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Ultrasound Med Biol. 2016 Feb;42(2):481-92. doi: 10.1016/j.ultrasmedbio.2015.10.009. Epub 2015 Nov 23.
The purpose of this study was to investigate the effects on the brain of multiple sessions of blood-brain barrier (BBB) disruption using focused ultrasound (FUS) in combination with micro-bubbles over a range of acoustic exposure levels. Six weekly sessions of FUS, using acoustical pressures between 0.66 and 0.80 MPa, were performed under magnetic resonance guidance. The success and degree of BBB disruption was estimated by signal enhancement of post-contrast T1-weighted imaging of the treated area. Histopathological analysis was performed after the last treatment. The consequences of repeated BBB disruption varied from no indications of vascular damage to signs of micro-hemorrhages, macrophage infiltration, micro-scar formations and cystic cavities. The signal enhancement on the contrast-enhanced T1-weighted imaging had limited value for predicting small-vessel damage. T2-weighted imaging corresponded well with the effects on histopathology and could be used to study treatment effects over time. This study demonstrates that repeated BBB disruption by FUS can be performed with no or limited damage to the brain tissue.
本研究的目的是调查在一系列声学暴露水平下，使用聚焦超声（FUS）结合微泡多次破坏血脑屏障（BBB）对大脑的影响。在磁共振引导下，每周进行6次FUS治疗，声压在0.66至0.80MPa之间。通过治疗区域的对比增强T1加权成像的信号增强来评估BBB破坏的成功与否及程度。在最后一次治疗后进行组织病理学分析。反复破坏BBB的后果各不相同，从无血管损伤迹象到出现微出血、巨噬细胞浸润、微瘢痕形成和囊腔等迹象。对比增强T1加权成像上的信号增强对预测小血管损伤的价值有限。T2加权成像与组织病理学效应吻合良好，可用于研究随时间变化的治疗效果。本研究表明，FUS反复破坏BBB可对脑组织造成无损伤或有限损伤。
