Yao Wen-Jian, Wang Yong-Lian, Lu Jian-Guo, Guo Ling, Qi Bo, Chen Zhi-Jun
Department of Thoracic Surgery, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
Int J Clin Exp Pathol. 2015 Sep 1;8(9):10868-74. eCollection 2015.
MicroRNAs (miRNAs) act as key regulators of multiple cancers. MicroRNA-506 (miR-506) functions as a tumor suppressor in various types of cancers. However, its role in esophageal cancer remains unclear. In our study, we found that miR-506 was significantly down-regulated in esophageal cancer tissues and cell lines. In vitro assay, our results showed that ectopic over-expression of miR-506 inhibited esophageal cancer cells proliferation, meanwhile, cells proliferation was promoted by miR-506 inhibition. In exploring mechanisms underlying the inhibitive role, we found that miR-506 significantly decreased the expression and transcription activity of cAMP responsive element binding protein 1 (CREB1). CREB1, tumor oncogene, exhibited significantly promote effect on esophageal cancer cell proliferation. Taken together, our data identify a new role of miR-506 in esophageal cancer involving CREB1 suppression.
微小RNA（miRNA）是多种癌症的关键调节因子。微小RNA-506（miR-506）在多种类型癌症中发挥肿瘤抑制作用。然而，其在食管癌中的作用仍不清楚。在我们的研究中，我们发现miR-506在食管癌组织和细胞系中显著下调。体外实验中，我们的结果表明，miR-506的异位过表达抑制了食管癌细胞的增殖，同时，miR-506抑制促进了细胞增殖。在探索其抑制作用的潜在机制时，我们发现miR-506显著降低了环磷酸腺苷反应元件结合蛋白1（CREB1）的表达和转录活性。CREB1作为肿瘤癌基因，对食管癌细胞增殖具有显著的促进作用。综上所述，我们的数据确定了miR-506在食管癌中涉及抑制CREB1的新作用。
