Li Jia, Chen Weian, Li Yu'an, Chen Ying, Ding Zhangna, Yang Dehao, Zhang Xu
Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University Wenzhou 32500, Zhejiang, China.
Department of Pediatric Orthopedics, The Second Affiliated Hospital of Wenzhou Medical University Wenzhou 32500, Zhejiang, China.
Int J Clin Exp Pathol. 2015 Sep 1;8(9):11149-56. eCollection 2015.
This study was to investigate the efficacy of olfactory ensheathing cell (OEC) transplantation on experimental autoimmune encephalomyelitis (EAE).
EAE models were established by guinea pig spinal cord homogenate (GPSCH) immunization in Lewis rats. OECs were purified and cultured from the olfactory nerve layer of SD rats, and then transplanted to the EAE models through the vena caudalis (Group A) or into the lateral cerebral ventricle (Group B). Neurological function scores and body weights were daily recorded following transplantation, and histological analysis was performed to assess the pathological changes in EAE rats.
Cultured cells mainly exhibited bipolar or tripolar morphology, and the majority of these cells were positive for NGFR p75 staining. Neurological function scoring and the body weight measurement showed that, OEC transplantation could significantly improve the performance of EAE rats, and similar results were observed for the transplantation through the vena caudalis and into the lateral cerebral ventricle. Moreover, the transplanted OECs accumulated to the lesions in the brains of EAE rats, in spite of the different transplantation approaches. However, no significant differences in histopathology (HE and LFB staining) were observed between the OEC-transplanted groups and the control group.
OEC transplantation could exert beneficial effects in the treatment of EAE, no matter which the cells were transplanted through the vena caudalis or into the lateral cerebral ventricle. Our findings might provide evidence for the clinical treatment of multiple sclerosis with cell transplantation.
本研究旨在探讨嗅鞘细胞（OEC）移植对实验性自身免疫性脑脊髓炎（EAE）的疗效。
通过用豚鼠脊髓匀浆（GPSCH）免疫Lewis大鼠建立EAE模型。从SD大鼠的嗅神经层纯化并培养OEC，然后通过尾静脉（A组）或注入侧脑室（B组）将其移植到EAE模型中。移植后每天记录神经功能评分和体重，并进行组织学分析以评估EAE大鼠的病理变化。
培养的细胞主要表现为双极或三极形态，并且这些细胞中的大多数NGFR p75染色呈阳性。神经功能评分和体重测量结果表明，OEC移植可显著改善EAE大鼠的表现，通过尾静脉和注入侧脑室移植均观察到类似结果。此外，尽管移植途径不同，但移植的OEC在EAE大鼠脑内的病变部位聚集。然而，OEC移植组与对照组之间在组织病理学（HE和LFB染色）方面未观察到显著差异。
无论通过尾静脉还是注入侧脑室移植细胞，OEC移植在治疗EAE中均可发挥有益作用。我们的研究结果可能为细胞移植治疗多发性硬化症提供临床依据。
