School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.
School of Chemistry, Biology, and Material Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China.
Bioresour Technol. 2016 Feb;201:8-14. doi: 10.1016/j.biortech.2015.11.039. Epub 2015 Nov 28.
A cellulase producing strain was newly isolated from soil samples and identified as Paenibacillus sp. LLZ1. A novel aqueous-dimethyl sulfoxide (DMSO)/1-ethyl-3-methylimidazolium diethyl phosphate ([Emin]DEP)-cellulase system was designed and optimized. In the pretreatment, DMSO was found to be a low-cost substitute of up to 70% ionic liquid to enhance the cellulose dissolution. In the enzymatic saccharification, the optimum pH and temperature of the Paenibacillus sp. LLZ1 cellulase were identified as 6.0 and 40°C, respectively. Under the optimized reaction condition, the conversion of microcrystalline cellulose and bagasse cellulose increased by 39.3% and 37.6%, compared with unpretreated cellulose. Compared to current methods of saccharification, this new approach has several advantages including lower operating temperature, milder pH, and less usage of ionic liquid, indicating a marked progress in environmental friendly hydrolysis of biomass-based materials.
一株产纤维素酶的菌株最近从土壤样本中分离出来，并被鉴定为解淀粉芽孢杆菌 LLZ1。设计并优化了一种新型的水-二甲基亚砜（DMSO）/1-乙基-3-甲基咪唑二乙基磷酸酯([Emin]DEP)-纤维素酶体系。在预处理中，发现 DMSO 可以替代高达 70%的离子液体，从而提高纤维素的溶解能力。在酶解糖化过程中，确定解淀粉芽孢杆菌 LLZ1 纤维素酶的最适 pH 值和温度分别为 6.0 和 40°C。在优化的反应条件下，与未经预处理的纤维素相比，微晶纤维素和蔗渣纤维素的转化率分别提高了 39.3%和 37.6%。与目前的糖化方法相比，这种新方法具有操作温度低、pH 值温和、离子液体用量少等优点，表明在生物质基材料的环保水解方面取得了显著进展。
