Rodríguez Carlos E, Palacios Javier, Fajardo Ignacio, Urdiales José Luis, Le Guével Xavier, Lozano José, Sánchez-Jiménez Francisca
Proteomics Unit of the Supercomputing and Bioinnovation Center of the University of Málaga, Parque Tecnológico de Andalucía, Málaga, Spain.
Department of Molecular Biology and Biochemistry, University of Malaga, Málaga, Spain.
J Am Soc Mass Spectrom. 2016 Feb;27(2):366-9. doi: 10.1007/s13361-015-1299-3. Epub 2015 Nov 30.
This is the first study where graphene is used as a MALDI adjuvant in combination with the traditional matrix α-cyano-4-hydroxycinnamic acid (CHCA) to improve the signal intensity of peptide samples. Use of this amended matrix not only leads to increased signals but also to a higher number of peaks detected in complex samples. Additionally, the use of graphene has a stabilizing effect that can also be exploited to improve the detection of easily cleavable molecules. Graphical Abstract ᅟ.
这是第一项将石墨烯用作基质辅助激光解吸电离（MALDI）佐剂，并与传统基质α-氰基-4-羟基肉桂酸（CHCA）结合使用以提高肽样品信号强度的研究。使用这种改良后的基质不仅会使信号增强，还会使在复杂样品中检测到的峰数量增加。此外，石墨烯的使用具有稳定作用，也可用于改善对易裂解分子的检测。图形摘要ᅟ。
