囊性纤维化成纤维细胞对哇巴因毒性的抗性增加。
Increased resistance of cystic fibrosis fibroblasts to ouabain toxicity.
作者信息
Epstein J, Breslow J L
出版信息
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1676-9. doi: 10.1073/pnas.74.4.1676.
Diploid skin fibroblasts derived from several unrelated patients with cystic fibrosis (CF) were tested for resistance to the cytotoxic effects of ouabain in comparison with cells from normal individuals. Cells from CF and normal individuals were plated at low density and exposed to ouabain at different concentrations for 24 hr. Dose-response curves showed that CF cells survived significantly better than did normal cells at all ouabain concentrations from 0.1 nM to 1 micronM in potassium-deficient medium. In medium containing the usual amount of potassium (6.25 mM), survival of the CF cells exposed to ouabain was decreased to that of the normal cells. If the glucose concentration of the potassium-deficient medium was decreased to 10% of the usual amount, the resistance of the CF cells to ouabain disappeared and their survival was identical to that of the normal cells. These findings may be useful in understanding the biochemical basis of CG and its clinical manifestations.
从几名患有囊性纤维化（CF）的无关患者身上获取的二倍体皮肤成纤维细胞，与正常个体的细胞相比，测试了其对哇巴因细胞毒性作用的抗性。将CF患者和正常个体的细胞低密度接种，并暴露于不同浓度的哇巴因中24小时。剂量反应曲线表明，在低钾培养基中，从0.1 nM到1 μM的所有哇巴因浓度下，CF细胞的存活情况明显优于正常细胞。在含有正常钾含量（6.25 mM）的培养基中，暴露于哇巴因的CF细胞的存活率降至正常细胞的水平。如果低钾培养基中的葡萄糖浓度降至正常量的10%，CF细胞对哇巴因的抗性消失，其存活率与正常细胞相同。这些发现可能有助于理解CF的生化基础及其临床表现。
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