Perrier F, François P, Rouhan D, Joannard A, Beaudoing A
Laboratoire de microbiologie, CHRU, Grenoble, France.
Pediatrie. 1989;44(3):221-6.
The diagnostic interest of the search for soluble bacterial antigens, using counter-current immunoelectrophoresis (CIE) has been evaluated in 109 children hospitalized with acute infection. In meningitis, CIE was well correlated with cerebrospinal fluid (CSF) culture and allowed a rapid diagnostic orientation in 82% of meningitis which were confirmed by classical bacteriology (CIE has to be performed using CSF and concentrated urine). False positive results were observed with type B meningococcus, especially on urine samples. In respiratory infections, the search for soluble antigens was of no interest except for focal pneumonitis; in that case, CIE was more frequently positive (35%) than blood culture (28%) and led to a 31% increase of correct diagnosis (CIE must be performed using concentrated urine). Serum and pleural fluid investigations were less sensitive. CIE was not useful in case of upper respiratory or nonfocal broncho-pulmonary infection, due to its very low efficiency.
采用对流免疫电泳（CIE）检测可溶性细菌抗原的诊断价值，已在109例因急性感染住院的儿童中进行了评估。在脑膜炎中，CIE与脑脊液（CSF）培养结果高度相关，在82%经传统细菌学确诊的脑膜炎病例中可实现快速诊断定位（CIE必须使用脑脊液和浓缩尿液进行检测）。B型脑膜炎球菌检测出现假阳性结果，尤其是在尿液样本中。在呼吸道感染中，除局灶性肺炎外，检测可溶性抗原并无意义；在此情况下，CIE阳性率（35%）高于血培养（28%），正确诊断率提高了31%（CIE必须使用浓缩尿液进行检测）。血清和胸水检测敏感性较低。由于效率极低，CIE在上呼吸道或非局灶性支气管肺部感染中并无用处。
