Borji Hassan, Haghparast Alireza, Soleimani Nooshinmehr, Azizzadeh Mohammad, Nazemshirazi Mohammad Hossein
Dept. of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Dept. of Clinical Science, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Iran J Parasitol. 2015 Jul-Sep;10(3):498-504.
Recognition of helminth-derived pathogen associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs), including toll like receptors (TLRs) is the first step towards initiating anti-helminth immune responses. Methods : Using somatic antigens of Ostertagia occidentalis, an important abomasal parasite of ruminants, the expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells (PBMCs) was analyzed by real-time quatitative reverse-transcription polymerase chain reaction (qRT-PCR). Somatic antigens of O. occidentalis were prepared to stimulate ovine PBMCs in a time and dose dependent manner. Results : A high expression of TLR2 and TLR4 was observed in PBMCs cultured with somatic antigens of the parasites specially when PBMCs were cultured with 100 µg/ml of somatic antigens and incubated for 2h. Up-regulation of TLR2 expression was more pronounced and evident in our study. Conclsusion : Somatic antigens of O. occidentalis have immunostimulatory and dominant role on peripheral immune cells. This study provide for the first time evidence of induction of TLRs in ovine PBMCs by somatic antigen of O. occidentalis.
模式识别受体（PRR）识别蠕虫来源的病原体相关分子模式（PAMP），包括 toll 样受体（TLR），是启动抗蠕虫免疫反应的第一步。方法：使用反刍动物重要皱胃寄生虫西方奥斯特线虫的体细胞抗原，通过实时定量逆转录聚合酶链反应（qRT-PCR）分析绵羊外周血单核细胞（PBMC）中 TLR2 和 TLR4 的表达。制备西方奥斯特线虫的体细胞抗原，以时间和剂量依赖性方式刺激绵羊 PBMC。结果：在用寄生虫体细胞抗原培养的 PBMC 中观察到 TLR2 和 TLR4 的高表达，特别是当 PBMC 用 100 µg/ml 的体细胞抗原培养并孵育 2 小时时。在我们的研究中，TLR2 表达的上调更为明显。结论：西方奥斯特线虫的体细胞抗原对外周免疫细胞具有免疫刺激和主导作用。本研究首次提供了西方奥斯特线虫体细胞抗原诱导绵羊 PBMC 中 TLRs 的证据。
