Derda Monika, Wojtkowiak-Giera Agnieszka, Kolasa-Wołosiuk Agnieszka, Kosik-Bogacka Danuta, Hadaś Edward, Jagodziński Paweł P, Wandurska-Nowak Elżbieta
Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701 Poznan, Poland.
Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701 Poznan, Poland.
Exp Parasitol. 2016 Jun;165:30-4. doi: 10.1016/j.exppara.2016.02.012. Epub 2016 Mar 3.
Toll-like receptors (TLRs) play a key role in the innate immune responses to a variety of pathogens including parasites. TLRs are among the most highly conserved in the evolution of the receptor family, localized mainly on cells of the immune system and on other cells such as lung cells. The aim of this study was to determine for the first time the expression of TLR2 and TLR4 in the lung of Acanthamoeba spp. infected mice using quantitative real-time polymerase chain reaction (Q-PCR) and immunohistochemical (IHC) staining. The Acanthamoeba spp. were isolated from a patient with Acanthamoeba keratitis (AK) (strain Ac 55) and from environmental samples of water from Malta Lake (Poznań, Poland - strain Ac 43). We observed a significantly increased level of expression of TLR2 as well as TLR4 mRNA from 2 to 30 days post Acanthamoeba infection (dpi) in the lungs of mice infected with Ac55 (KP120880) and Ac43 (KP120879) strains. According to our observations, increased TLR2 and TLR4 expression in the pneumocytes, interstitial cells and epithelial cells of the bronchial tree may suggest an important role of these receptors in protective immunity against Acanthamoeba infection in the lung. Moreover, increased levels of TLR2 and TLR4 mRNA expression in infected Acanthamoeba mice may suggest the involvement of these TLRs in the recognition of this amoeba pathogen-associated molecular pattern (PAMP).
Toll样受体（TLRs）在包括寄生虫在内的多种病原体的天然免疫反应中发挥关键作用。TLRs是受体家族进化过程中最保守的成员之一，主要定位于免疫系统细胞以及其他细胞，如肺细胞。本研究的目的是首次使用定量实时聚合酶链反应（Q-PCR）和免疫组织化学（IHC）染色法，确定棘阿米巴属感染小鼠肺组织中TLR2和TLR4的表达情况。棘阿米巴属菌株分别从一名棘阿米巴角膜炎（AK）患者（菌株Ac 55）以及波兰波兹南马尔他湖的水样环境样本（菌株Ac 43）中分离得到。我们观察到，感染Ac55（KP120880）和Ac43（KP120879）菌株的小鼠肺部，在棘阿米巴感染后2至30天（dpi），TLR2以及TLR4 mRNA的表达水平显著升高。根据我们的观察，支气管树的肺细胞、间质细胞和上皮细胞中TLR2和TLR4表达增加，可能表明这些受体在肺部针对棘阿米巴感染的保护性免疫中发挥重要作用。此外，感染棘阿米巴的小鼠中TLR2和TLR4 mRNA表达水平升高，可能表明这些TLRs参与了对这种阿米巴病原体相关分子模式（PAMP）的识别。
