Radiosensitivity in HeLa cervical cancer cells overexpressing glutathione S-transferase π 1.

The aims of the present study were to investigate the effect of overexpressed exogenous glutathione S-transferase π 1 () gene on the radiosensitivity of the HeLa human cervical cancer cell line and conduct a preliminarily investigation into the underlying mechanisms of the effect. The full-length sequence of human was obtained by performing a polymerase chain reaction (PCR) using primers based on the GenBank sequence of Subsequently, the gene was cloned into a recombinant eukaryotic expression plasmid, and the resulting construct was confirmed by restriction analysis and DNA sequencing. A HeLa cell line that was stably expressing high levels of was obtained through stable transfection of the constructed plasmids using lipofectamine and screening for G418 resistance, as demonstrated by reverse transcription-PCR. Using the transfected HeLa cells, a colony formation assay was conducted to detect the influence of overexpression on the cell radiosensitivity. Furthermore, flow cytometry was used to investigate the effect of overexpression on cell cycle progression, with the protein expression levels of the cell cycle regulating factor cyclin B1 detected using western blot analysis. Colony formation and G/M phase arrest in the -expressing cells were significantly increased compared with the control group (P<0.01). In addition, the expression of cyclin B1 was significantly reduced in the -expressing cells. These results demonstrated that increased expression of inhibits radiosensitivity in HeLa cells. The mechanism underlying this effect may be associated with the ability of the GSTP1 protein to reduce cyclin B1 expression, resulting in significant G/M phase arrest.