Alshatwi Ali A, Athinarayanan Jegan, Vaiyapuri Subbarayan Periasamy
Nanobiotechnology and Molecular Biology Research Laboratory, Department of Food Science and Nutrition, College of Food Science and Agriculture, King Saud University, P.O. Box 2460, Riyadh, 11451, Kingdom of Saudi Arabia,
J Mater Sci Mater Med. 2015 Jan;26(1):5330. doi: 10.1007/s10856-014-5330-1. Epub 2015 Jan 11.
Platinum-based chemotherapeutic drugs, including cisplatin, carboplatin, and oxaliplatin, have been used to manage cancer in spite of dose-dependent side effects, including nephrotoxicity, neurotoxicity and ototoxicity. These disadvantages have prompted the development of new strategies for cancer therapy that utilize functionalized nanoparticles as nanomedicines. In the present investigation, we have synthesized platinum nanoparticles using tea polyphenol (TPP) as both a reducing and surface modifying agent. The crystalline nature and morphology of the prepared TPP-functionalized platinum nanoparticles (TPP@Pt) were analyzed using X-ray diffraction (XRD) and transmission electron microscopy (TEM). The XRD results revealed that the TPP@Pt had a crystalline nature with a face-centered cubic structure. TEM imaging suggested that the TTP@Pt are flower shaped with a well-dispersed 30-60 nm-sized TPP@Pt formation. Cervical cancer cells (SiHa) were then treated with different concentrations of TPP@Pt. The effects of TPP@Pt on cell viability, nuclear morphology and cell cycle distribution were investigated. A cell viability assay revealed that the proliferation of SiHa cells was inhibited by TPP@Pt. Propidium iodide nuclear staining indicated that TPP@Pt induced nuclear fragmentation and chromatin condensation. Treatment with TPP@Pt significantly increased the percentage of cells in the G2/M phase, which indicates induced cell cycle arrest in the G2/M phase and an increased number of cells in the subG0 cell death phase. These findings highlight a potential use of TPP@Pt in cervical cancer treatment.
包括顺铂、卡铂和奥沙利铂在内的铂类化疗药物，尽管存在剂量依赖性副作用，如肾毒性、神经毒性和耳毒性，但仍被用于治疗癌症。这些缺点促使人们开发新的癌症治疗策略，利用功能化纳米颗粒作为纳米药物。在本研究中，我们使用茶多酚（TPP）作为还原剂和表面改性剂合成了铂纳米颗粒。使用X射线衍射（XRD）和透射电子显微镜（TEM）分析了制备的TPP功能化铂纳米颗粒（TPP@Pt）的晶体性质和形态。XRD结果表明，TPP@Pt具有面心立方结构的晶体性质。TEM成像表明，TPP@Pt呈花状，形成了尺寸为30 - 60nm且分散良好的TPP@Pt。然后用不同浓度的TPP@Pt处理宫颈癌细胞（SiHa）。研究了TPP@Pt对细胞活力、核形态和细胞周期分布的影响。细胞活力测定表明，TPP@Pt抑制了SiHa细胞的增殖。碘化丙啶核染色表明，TPP@Pt诱导了核碎片化和染色质浓缩。用TPP@Pt处理显著增加了G2/M期细胞的百分比，这表明诱导细胞周期停滞在G2/M期，并且亚G0期细胞死亡阶段的细胞数量增加。这些发现突出了TPP@Pt在宫颈癌治疗中的潜在用途。
