Kimura Yusuke, Ishikawa Masashi, Hori Yoko, Okabe Tadashi, Sakamoto Atsuhiro
Department of Anesthesiology and Pain Medicine, Graduate School of Medicine, Nippon Medical School, Tokyo 113-8603, Japan.
Department of Anesthesiology, Hitachi, Ltd., Hitachinaka General Hospital, Ibaraki 312-0057, Japan.
Biomed Rep. 2015 Nov;3(6):802-806. doi: 10.3892/br.2015.525. Epub 2015 Sep 30.
Previous reports have shown that electroconvulsive therapy (ECT) is efficacious in the treatment of neuropathic pain; however, its mechanism of action remains unclear. The present study aimed to understand these mechanisms by investigating the alterations in the expression of neuropeptide Y () and interleukin- () in the prefrontal cortex. A rat model of neuropathic pain produced by chronic constrictive injury of the sciatic nerve was used, and mechanical and thermal hyperalgesia were evaluated starting 2 days after the injury. Using a pulse generator, ECT was administered to the rodents for 6 days from days 7-12 after the injury. Thermal and mechanical stimulation were administered to assess pain thresholds. Quantitative polymerase chain reaction, used to measure gene expression levels in the prefrontal cortex, showed that and gene expression levels in the prefrontal cortex increased following the injury. The present results indicate that these gene expression level variations may be associated with the mechanisms underlying the effect of ECT in treating neuropathic pain.
先前的报告表明，电休克疗法（ECT）在治疗神经性疼痛方面是有效的；然而，其作用机制仍不清楚。本研究旨在通过调查前额叶皮质中神经肽Y（NPY）和白细胞介素-6（IL-6）表达的变化来了解这些机制。使用坐骨神经慢性压迫损伤产生的神经性疼痛大鼠模型，并在损伤后2天开始评估机械性和热性痛觉过敏。使用脉冲发生器，在损伤后第7 - 12天对啮齿动物进行6天的ECT治疗。给予热刺激和机械刺激以评估疼痛阈值。用于测量前额叶皮质中基因表达水平的定量聚合酶链反应表明，损伤后前额叶皮质中NPY和IL-6基因表达水平增加。目前的结果表明，这些基因表达水平的变化可能与ECT治疗神经性疼痛的作用机制有关。
