Rashed Reham A, Kadry Dalia Y, El Taweel Maha, Abd El Wahab Nahed, Abd El Hameed Thoreya
Clinical Pathology, Medicine, National Cancer Institute, Cairo, Egypt E-mail :
Asian Pac J Cancer Prev. 2015;16(17):7875-82. doi: 10.7314/apjcp.2015.16.17.7875.
The objectives of this study were to evaluate the expression of brain and acute leukemia, cytoplasmic (BAALC) gene and erythroblast transformation-specific related gene (ERG) in de novo cases of acute myeloid leukemia (AML) and identify roles in disease progression and outcome.
This study included 50 newly diagnosed AML patients, along with 10 apparently healthy normal controls. BAALC and ERG expression was detected in the bone marrow of both patients and controls using real-time RT-PCR.
BAALC and ERG expression was detected in 52% of cases but not in any controls. There was a statistically significant correlation between BAALC and ERG gene expression and age (p- value=0.004 and 0.019, respectively). No statistical significance was noted for sex, lymphadenopathy, hepatomegaly, splenomegaly, other hematological findings, immunophenotyping and FAB sub-classification except for ERG gene and FAB (p-value=0.058). A statistical significant correlation was found between response to treatment with ERG expression (p-value=0.028) and age (p-value=0.014). A statistically significant variation in overall survival was evident with patient age, BM blast cells, FAB subgroups, BAALC and ERG expression (p-value= <0.001, 0.045, 0.041, <0.008 and 0.025 respectively).
Our results suggest that BAALC and ERG genes are specific significant molecular markers in AML disease progression, response to treatment and survival.
本研究的目的是评估脑和急性白血病、细胞质（BAALC）基因及成红细胞转化特异性相关基因（ERG）在急性髓系白血病（AML）初发病例中的表达情况，并确定其在疾病进展和预后中的作用。
本研究纳入50例新诊断的AML患者以及10例表面健康的正常对照。采用实时逆转录聚合酶链反应（RT-PCR）检测患者和对照骨髓中的BAALC和ERG表达。
52%的病例检测到BAALC和ERG表达，而对照中均未检测到。BAALC和ERG基因表达与年龄之间存在统计学显著相关性（p值分别为0.004和0.019）。除ERG基因与FAB（p值 = 0.058）外，性别、淋巴结病、肝肿大、脾肿大、其他血液学检查结果、免疫表型分析及FAB亚分类均无统计学意义。发现ERG表达与治疗反应（p值 = 0.028）及年龄（p值 = 0.014）之间存在统计学显著相关性。患者年龄、骨髓原始细胞、FAB亚组、BAALC和ERG表达在总生存方面存在统计学显著差异（p值分别为<0.001、0.045、0.041、<0.008和0.025）。
我们的结果表明，BAALC和ERG基因是AML疾病进展、治疗反应及生存中的特异性重要分子标志物。
