Mehralizadeh Hossein, Aliparasti Mohammad Reza, Talebi Mehdi, Salekzamani Shabnam, Almasi Shohreh, Raeisi Morteza, Yousefi Mehdi, Movassaghpour AliAkbar
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Pharm Bull. 2021 Jan;11(1):197-203. doi: 10.34172/apb.2021.021. Epub 2020 Nov 7.
Acute myeloid leukemia (AML) is the most prevalent acute leukemia in adults. It possesses different cytogenetic and molecular features. The expression of Wilms tumor-1 , brain and acute leukemia, cytoplasmic and ETS-related gene might be considered as prognostic factors in AML patients. The aim of this study was to determine the mRNA expressions of , and genes in bone marrow of mononuclear cells and their effects on complete remission in the Iranian AML patients, pre- and post- chemotherapy. Forty AML patients with normal karyotype were evaluated. The mRNA gene expressions were measured with quantitative real-time PCR in bone marrow of mononuclear cells of AML patients at the baseline and after chemotherapy. The subtypes of AML and flow cytometry panel were also assessed. Complete remission (CR) after the treatment was addressed for all patients. The mRNA expressions of and were significantly decreased after the treatment ( = 0.001, 0.017, 0.036). mRNA expression was inversely correlated with CR after chemotherapy ( =0.024). There was also significant correlation between baseline expression of BAALC and CR ( =0.046). No significant correlation was observed between ERG and CR pre- and post- chemotherapy ( =0.464 and 0.781). There was also significant correlation between mRNA expression and CD34+ ( <0.001). The present study showed that decreased significantly after standard chemotherapy which could have favorable effects on CR. Also, the high expression of could have a poor prognostic role in AML patients. The identification of these gene expressions can be an efficient approach in targeted therapy among AML patients.
急性髓系白血病（AML）是成人中最常见的急性白血病。它具有不同的细胞遗传学和分子特征。肾母细胞瘤-1、脑和急性白血病、细胞质和ETS相关基因的表达可能被视为AML患者的预后因素。本研究的目的是确定伊朗AML患者化疗前后骨髓单个核细胞中、和基因的mRNA表达及其对完全缓解的影响。对40例核型正常的AML患者进行了评估。采用定量实时PCR检测AML患者骨髓单个核细胞在基线和化疗后的mRNA基因表达。还评估了AML的亚型和流式细胞术检测结果。研究了所有患者治疗后的完全缓解（CR）情况。治疗后和的mRNA表达显著降低（=0.001、0.017、0.036）。化疗后mRNA表达与CR呈负相关（=0.024）。BAALC的基线表达与CR之间也存在显著相关性（=0.046）。化疗前后ERG与CR之间未观察到显著相关性（=0.464和0.781）。mRNA表达与CD34+之间也存在显著相关性（<0.001）。本研究表明，标准化疗后显著降低，这可能对CR有有利影响。此外，的高表达可能在AML患者中具有不良预后作用。识别这些基因表达可能是AML患者靶向治疗的有效方法。
