Sun Pengfei, Hui Cai, Bai Naling, Yang Shengmao, Wan Li, Zhang Qichun, Zhao YuHua
College of Life Sciences, Zhejiang University, 310058 Hangzhou, Zhejiang, PR China.
Institute of Environment Resource and Soil Fertilizer, Zhejiang Academy of Agriculture Science, 310021 Hangzhou, Zhejiang, China.
Sci Rep. 2015 Dec 2;5:17465. doi: 10.1038/srep17465.
In the present work, a novel bioflocculant, EPS-1, was prepared and used to flocculate the kaolin suspension and Microcystis aeruginosa. We focused on the characteristics and flocculation performance of EPS-1, especially with regard to its protein components. An important attribute of EPS-1 was its protein content, with 18 protein types identified that occupied a total content of 31.70% in the EPS-1. Moreover, the flocculating activity of these protein components was estimated to be no less than 33.93%. Additionally, polysaccharides that occupied 57.12% of the total EPS-1 content consisted of four monosaccharides: maltose, D-xylose, mannose, and D-fructose. In addition, carbonyl, amino, and hydroxyl groups were identified as the main functional groups. Three main elements, namely C1s, N1s, and O1s, were present in EPS-1 with relative atomic percentages of 62.63%, 24.91%, and 10.5%, respectively. Zeta potential analysis indicated that charge neutralization contributed to kaolin flocculation, but was not involved in M. aeruginosa flocculation. The flocculation conditions of EPS-1 were optimized, and the maximum flocculating efficiencies were 93.34% within 2 min for kaolin suspension and 87.98% within 10 min for M. aeruginosa. These results suggest that EPS-1 could be an alternative to chemical flocculants for treating wastewaters and cyanobacterium-polluted freshwater.
在本研究中，制备了一种新型生物絮凝剂EPS-1，并将其用于絮凝高岭土悬浮液和铜绿微囊藻。我们重点研究了EPS-1的特性和絮凝性能，特别是其蛋白质成分。EPS-1的一个重要特性是其蛋白质含量，共鉴定出18种蛋白质类型，占EPS-1总含量的31.70%。此外，这些蛋白质成分的絮凝活性估计不低于33.93%。另外，占EPS-1总含量57.12%的多糖由四种单糖组成：麦芽糖、D-木糖、甘露糖和D-果糖。此外，羰基、氨基和羟基被确定为主要官能团。EPS-1中存在三种主要元素，即C1s、N1s和O1s，其相对原子百分比分别为62.63%、24.91%和10.5%。zeta电位分析表明，电荷中和有助于高岭土的絮凝，但不参与铜绿微囊藻的絮凝。对EPS-1的絮凝条件进行了优化，高岭土悬浮液在2分钟内的最大絮凝效率为93.34%，铜绿微囊藻在10分钟内的最大絮凝效率为87.98%。这些结果表明，EPS-1可作为化学絮凝剂的替代品，用于处理废水和蓝藻污染的淡水。
