College of Life Sciences, Zhejiang University, 310058 Hangzhou, Zhejiang, PR China.
Institute of Environment Resource and Soil Fertilizer, Zhejiang Academy of Agriculture Science, Hangzhou 310021, PR China.
J Hazard Mater. 2015 Mar 2;284:215-21. doi: 10.1016/j.jhazmat.2014.11.025. Epub 2014 Nov 20.
A novel composite consisting of clay, bioflocculant, and inorganic flocculant was designed, and its flocculating effect on harmful algal blooms (HABs) was studied in this study. The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite. The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity. The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L. The flocculating rates of the composite were tested, and it could rapidly reach 100±0.13% within 2 min when OD680 of Microcystis aeruginosa was 0.1; it could reach 100±0.08% within 5 min for OD680 of M. aeruginosa in HABs up to 1.0. These results suggest that the novel composite will be a highly efficient material for the treatment of HABs caused by M. aeruginosa.
本研究设计了一种由粘土、生物絮凝剂和无机絮凝剂组成的新型复合材料，并研究了其对有害藻华（HAB）的絮凝效果。由新分离的铜绿假单胞菌 ZJU1 产生的胞外聚合物物质（EPS），产量为 3.58±0.11 g/L，被认为是复合材料的关键组成部分。分析了 EPS 的成分和官能团，结果表明多糖、蛋白质和核酸是其主要成分；EPS 中的极性官能团负责其絮凝活性。通过响应面法对新型复合材料进行了优化，优化后，复合材料的光学成分和含量为高岭土 2.38 g/L、CaCl2 0.28 g/L、KAl(SO4)2 0.09 g/L 和 EPS 1.75 mg/L。测试了复合材料的絮凝率，当铜绿微囊藻的 OD680 为 0.1 时，在 2 分钟内可迅速达到 100±0.13%；当 HAB 中铜绿微囊藻的 OD680 达到 1.0 时，在 5 分钟内可达到 100±0.08%。这些结果表明，该新型复合材料将成为一种高效的铜绿微囊藻引起的 HAB 处理材料。
