Gao Dongmei, Zhao Jun, Li Xiuyi, Xia Yuanyuan, Cai Yu, Pan Jiwen, Zhou Hong, Fang Yong, Zhang Shouzhu, Wen He
Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
Department of Microbiology, Anhui Medical University 69 Meishan Road, Hefei 230032, China.
Int J Clin Exp Med. 2015 Sep 15;8(9):14999-5004. eCollection 2015.
The purpose of the present study was to determine whether type III IFN can modulate the autophagic response in human osteosarcoma cell.
Human osteosarcoma cell were treated with Interferon-λ1. We investigated that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells by Matrigel invasion assay. Autophagy were assessed by acridine orange staining, MDC staining and Transmission electron microscopy.
In this study, we found that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Acridine orange staining and MDC staining showed that Interferon-λ1 triggered the accumulation of acidic vesicular and autolysosomes in osteosarcoma cell. The acridine orange osteosarcoma cell ratios were 3.6 ± 0.5%, 4.5 ± 0.8%, and 12.4 ± 1.7% after treatment with 1, 10, and 100 ng/mL Interferon-λ1 for 48 h. Osteosarcoma cell cells treated with 100 ng/mL Interferon-λ1 for 48 h developed autophagy some-like characteristics, including single or double-membrane vacuoles containing intact and degraded cellular debris.
Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Autophagy can be induced in a dose-dependent manner by treatment with Interferon-λ1 in osteosarcoma cell.
本研究旨在确定III型干扰素是否能调节人骨肉瘤细胞中的自噬反应。
用人骨肉瘤细胞与干扰素λ1进行处理。我们通过基质胶侵袭试验研究了干扰素λ1是否能抑制骨肉瘤细胞的侵袭能力。通过吖啶橙染色、MDC染色和透射电子显微镜评估自噬情况。
在本研究中，我们发现干扰素λ1能抑制骨肉瘤细胞的侵袭能力。吖啶橙染色和MDC染色显示，干扰素λ1能促使骨肉瘤细胞中酸性囊泡和自溶酶体的积累。用1、10和100 ng/mL干扰素λ1处理48小时后，吖啶橙染色的骨肉瘤细胞比例分别为3.6±0.5%、4.5±0.8%和12.4±1.7%。用100 ng/mL干扰素λ1处理48小时的骨肉瘤细胞呈现出一些自噬样特征，包括含有完整和降解细胞碎片的单膜或双膜空泡。
干扰素λ1能抑制骨肉瘤细胞的侵袭能力。在骨肉瘤细胞中，用干扰素λ1处理可剂量依赖性地诱导自噬。
