Wang Chen, Ju Hong, Shen Chunyan, Tong Zhongsheng
Department of Breast Oncology; Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education; Key Laboratory of Cancer Prevention and Therapy, Tianjin; Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer Tianjin 300060, China.
Tianjin Eye Hospital Tianjin 300020, China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15648-56. eCollection 2015.
Vitamin E δ-tocotrienol has been reported to possess anticancer activity both in vitro and in vivo. However, the underlying molecular mechanisms of δ-tocotrienol induced apoptosis in triple-negative breast cancer are not fully understood. Here, we reported that microRNA-429 (miR-429) is up-regulated in two TNBC cell lines (MDA-MB-231 and MDA-MB-468), treated with δ-tocotrienol. Inhibition of miR-429 may partially rescue the apoptosis induced by δ-tocotrienol in MDA-MB-231 cells. We also showed that the forced expression of miR-429 was sufficient to lead to apoptosis in MDA-MB-231 cells. Furthermore, we identified X-linked inhibitor of apoptosis protein (XIAP) as one of miR-429's target genes. These results suggest that the activation of miR-429 by δ-tocotrienol may be an effective approach for the prevention and treatment of triple-negative breast cancer.
据报道，维生素E δ-生育三烯酚在体外和体内均具有抗癌活性。然而，δ-生育三烯酚诱导三阴性乳腺癌细胞凋亡的潜在分子机制尚未完全明确。在此，我们报道，在用δ-生育三烯酚处理的两种三阴性乳腺癌细胞系（MDA-MB-231和MDA-MB-468）中，微小RNA-429（miR-429）上调。抑制miR-429可能部分挽救δ-生育三烯酚在MDA-MB-231细胞中诱导的凋亡。我们还表明，miR-429的强制表达足以导致MDA-MB-231细胞凋亡。此外，我们确定X连锁凋亡抑制蛋白（XIAP）是miR-429的靶基因之一。这些结果表明，δ-生育三烯酚激活miR-429可能是预防和治疗三阴性乳腺癌的有效方法。
