Amasyali Akin Soner, Akkurt Abdullah, Kazan Ercan, Yilmaz Mustafa, Erol Bulent, Yildiz Yuksel, Erol Haluk
Department of Urology, School of Medicine, Adnan Menderes University Aydin, Turkey.
Department of Biochemistry, School of Medicine, Adnan Menderes University Aydin, Turkey.
Int J Clin Exp Med. 2015 Sep 15;8(9):15766-72. eCollection 2015.
To investigate the effect of the tadalafil in experimental renal I/R injury and to evaluate these changes with IMA (nonspesific early biomarker of ischemia), NO and MDA levels.
Twenty four female Wistar rats were randomly divided into 3 groups (n=8): Group I, sham; Group II, 60 min I/R; Group III, 60 min I/R plus tadalafil. Tadalafil was administered via an orogastric tube (10 mg/kg) 24 h prior to the procedure. After ischemia of the left kidney and 1 h of reperfusion, blood samples were obtained, and the kidney was removed.
Statistically significant histopathologic changes were exist between groups, with the most severe injury was determined in group II in comparison to the others (X(2)=21,803, P=0.000). Also mean serum IMA levels were higher in group II, but not statistically significant (19.83±7.81 U/ml, 22.26±7.14 U/ml and 19.82±7.77 U/ml, P=0.613). In addition, NO values were lower in I/R groups (P=0.049). There were no differences among the groups in terms of MDA.
IMA may be used as a nonselective biomarker for IR injury before the occurrence of necrosis. Decreased IMA levels may indicate the nephroprotective effect of tadalafil in renal IR injury.
研究他达拉非对实验性肾缺血/再灌注损伤的影响，并通过缺血修饰白蛋白（IMA，一种非特异性早期缺血生物标志物）、一氧化氮（NO）和丙二醛（MDA）水平评估这些变化。
24只雌性Wistar大鼠随机分为3组（每组n = 8）：I组，假手术组；II组，60分钟缺血/再灌注组；III组，60分钟缺血/再灌注加他达拉非组。在手术前24小时通过胃管给予他达拉非（10毫克/千克）。左肾缺血1小时后再灌注，采集血样并取出肾脏。
各组之间存在统计学上显著的组织病理学变化，与其他组相比，II组损伤最为严重（X(2)=21.803，P = 0.000）。此外，II组血清IMA平均水平较高，但无统计学意义（分别为19.83±7.81 U/ml、22.26±7.14 U/ml和19.82±7.77 U/ml，P = 0.613）。另外，缺血/再灌注组的NO值较低（P = 0.049）。各组之间MDA水平无差异。
IMA可作为坏死发生前缺血/再灌注损伤的非选择性生物标志物。IMA水平降低可能表明他达拉非对肾缺血/再灌注损伤具有肾保护作用。
