伊拉克患者中防御素β-4基因拷贝数增加与白塞病的关联
Association of Higher Defensin β-4 Genomic Copy Numbers with Behçet's Disease in Iraqi Patients.
作者信息
Hameed Ammar F, Jaradat Sameh, Al-Musawi Bassam M, Sharquie Khalifa, Ibrahim Mazin J, Hayani Raafa K, Norgauer Johannes
机构信息
Departments of Dermatology, College of Medicine, University of Baghdad, Baghdad, Iraq;
Department of Dermatology, Jena University Hospital, Jena, Germany;
出版信息
Sultan Qaboos Univ Med J. 2015 Nov;15(4):e491-5. doi: 10.18295/squmj.2015.15.04.008. Epub 2015 Nov 23.
OBJECTIVES
Behçet's disease (BD) is an immune-mediated small vessel systemic vasculitis. Human β-defensins are antimicrobial peptides associated with many inflammatory diseases and are encoded by the β-defensin family of multiple-copy genes. However, their role in BD necessitates further investigation. The aim of the present study was to investigate the possible association of BD in its various clinical forms with defensin β-4 (DEFB4) genomic copy numbers.
METHODS
This case-control study was conducted from January to September 2011 and included 50 control subjects and 27 unrelated Iraqi BD patients registered at Baghdad Teaching Hospital, Bagdad, Iraq. Copy numbers of the DEFB4 gene were determined using the comparative cycle threshold method by duplex real-time polymerase chain reaction technology at the Department of Dermatology of Jena University Hospital, Jena, Germany.
RESULTS
DEFB4 genomic copy numbers were significantly higher in the BD group compared to the control group (P = 0.010). However, no statistically significant association was found between copy numbers and clinical variables within the BD group.
CONCLUSION
The DEFB4 copy number polymorphism may be associated with BD; however, it is not associated with different clinical manifestations of the disease.
目的
白塞病（BD）是一种免疫介导的小血管系统性血管炎。人β-防御素是与多种炎症性疾病相关的抗菌肽，由多拷贝基因的β-防御素家族编码。然而，它们在BD中的作用需要进一步研究。本研究的目的是调查BD各种临床形式与防御素β-4（DEFB4）基因拷贝数之间可能存在的关联。
方法
本病例对照研究于2011年1月至9月进行，纳入了50名对照受试者和27名在伊拉克巴格达巴格达教学医院登记的无血缘关系的伊拉克BD患者。德国耶拿大学医院皮肤科使用比较循环阈值法通过双链实时聚合酶链反应技术测定DEFB4基因的拷贝数。
结果
与对照组相比，BD组的DEFB4基因拷贝数显著更高（P = 0.010）。然而，在BD组内，拷贝数与临床变量之间未发现统计学上的显著关联。
结论
DEFB4拷贝数多态性可能与BD相关；然而，它与该疾病的不同临床表现无关。
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