Vasani Roshan B, Janardanan Nayana, Prieto-Simón Beatriz, Cifuentes-Rius Anna, Bradley Siobhan J, Moore Eli, Kraus Tobias, Voelcker Nicolas H
ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
ACS Appl Mater Interfaces. 2015 Dec 23;7(50):27755-64. doi: 10.1021/acsami.5b08765. Epub 2015 Dec 14.
We demonstrate microwave-induced heating of gold nanoparticles and nanorods. An appreciably higher and concentration-dependent microwave-induced heating rate was observed with aqueous dispersions of the nanomaterials as opposed to pure water and other controls. Grafted with the thermoresponsive polymer poly(N-isopropylacrylamide), these gold nanomaterials react to microwave-induced heating with a conformational change in the polymer shell, leading to particle aggregation. We subsequently covalently immobilize concanavalin A (Con A) on the thermoresponsive gold nanoparticles. Con A is a bioreceptor commonly used in bacterial sensors because of its affinity for carbohydrates on bacterial cell surfaces. The microwave-induced thermal transitions of the polymer reversibly switch on and off the display of Con A on the particle surface and hence the interactions of the nanomaterials with carbohydrate-functionalized surfaces. This effect was determined using linear sweep voltammetry on a methyl-α-d-mannopyranoside-functionalized electrode.
我们展示了微波诱导的金纳米颗粒和纳米棒的加热过程。与纯水和其他对照相比，在纳米材料的水分散体中观察到明显更高且与浓度相关的微波诱导加热速率。这些金纳米材料接枝了热响应性聚合物聚（N-异丙基丙烯酰胺），它们对微波诱导的加热做出反应，聚合物壳发生构象变化，导致颗粒聚集。我们随后将伴刀豆球蛋白A（Con A）共价固定在热响应性金纳米颗粒上。Con A是一种生物受体，由于其对细菌细胞表面碳水化合物的亲和力，常用于细菌传感器。聚合物的微波诱导热转变可逆地开启和关闭Con A在颗粒表面的展示，从而影响纳米材料与碳水化合物功能化表面的相互作用。这种效应是通过在甲基-α-D-甘露吡喃糖苷功能化电极上使用线性扫描伏安法测定的。
