Hui Subhra Prakash, Nag Tapas Chandra, Ghosh Sukla
Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92, A. P. C. Road, Kolkata-700009, India.
Department of Anatomy, All India Institute of Medical Sciences, New Delhi- 110029, India.
PLoS One. 2015 Dec 2;10(12):e0143595. doi: 10.1371/journal.pone.0143595. eCollection 2015.
Zebrafish can repair their injured brain and spinal cord after injury unlike adult mammalian central nervous system. Any injury to zebrafish spinal cord would lead to increased proliferation and neurogenesis. There are presences of proliferating progenitors from which both neuronal and glial loss can be reversed by appropriately generating new neurons and glia. We have demonstrated the presence of multiple progenitors, which are different types of proliferating populations like Sox2+ neural progenitor, A2B5+ astrocyte/ glial progenitor, NG2+ oligodendrocyte progenitor, radial glia and Schwann cell like progenitor. We analyzed the expression levels of two common markers of dedifferentiation like msx-b and vimentin during regeneration along with some of the pluripotency associated factors to explore the possible role of these two processes. Among the several key factors related to pluripotency, pou5f1 and sox2 are upregulated during regeneration and associated with activation of neural progenitor cells. Uncovering the molecular mechanism for endogenous regeneration of adult zebrafish spinal cord would give us more clues on important targets for future therapeutic approach in mammalian spinal cord repair and regeneration.
与成年哺乳动物的中枢神经系统不同，斑马鱼在受伤后能够修复其受损的大脑和脊髓。斑马鱼脊髓的任何损伤都会导致增殖和神经发生增加。存在增殖祖细胞，通过适当地产生新的神经元和神经胶质细胞，可以逆转神经元和神经胶质细胞的损失。我们已经证明存在多种祖细胞，它们是不同类型的增殖群体，如Sox2 +神经祖细胞、A2B5 +星形胶质细胞/神经胶质祖细胞、NG2 +少突胶质细胞祖细胞、放射状胶质细胞和雪旺细胞样祖细胞。我们分析了再生过程中去分化的两个常见标志物msx - b和波形蛋白的表达水平，以及一些多能性相关因子，以探讨这两个过程的可能作用。在与多能性相关的几个关键因子中，pou5f1和sox2在再生过程中上调，并与神经祖细胞的激活有关。揭示成年斑马鱼脊髓内源性再生的分子机制将为我们提供更多关于哺乳动物脊髓修复和再生未来治疗方法重要靶点的线索。
