Morita T, Iwamoto Y, Shimizu T, Masuzawa T, Yanagihara Y
Chem Pharm Bull (Tokyo). 1989 Feb;37(2):407-9. doi: 10.1248/cpb.37.407.
The mutagenicity (Trp+ reversion) of procarcinogens such as N-nitrosodimethylamine, 3,4-benzpyrene and 2-acetylaminofluorene has been detected with the permeable mutant of yeast, Saccharomyces cerevisiae C658-K42. The original strain C658, however, showed a positive response only to N-nitrosodimethylamine with S9 mix. The permeable mutant C658-K42 was employed for mutagenicity tests on 12 carcinogens which had been reported to be non-mutagenic in Salmonella/microsome tests. It was found that phenobarbital, thiourea, 1,2-dimethylhydrazine and 4-aminoantipyrine were mutagenic in the presence of S9 mix. Benzene, o-toluidine and thioacetamide were weakly mutagenic. Negative results were obtained for diethylstilbestrol, safrole, acetamide, urethane and ethionine at the concentrations used. Caprolactam did not show any mutagenic effect on C658-K42 at concentrations up to 20 mg/ml. Sodium azide, which is unlikely to be carcinogenic but is strongly mutagenic in the Ames test, showed a very weak mutagenic effect on C658-K42.
已使用酵母酿酒酵母C658-K42的可渗透突变体检测了N-亚硝基二甲胺、3,4-苯并芘和2-乙酰氨基芴等前致癌物的诱变性（色氨酸+回复突变）。然而，原始菌株C658仅在加入S9混合物时对N-亚硝基二甲胺呈现阳性反应。使用可渗透突变体C658-K42对12种据报道在沙门氏菌/微粒体试验中无诱变性的致癌物进行了诱变性测试。结果发现，苯巴比妥、硫脲、1,2-二甲基肼和4-氨基安替比林在加入S9混合物时具有诱变性。苯、邻甲苯胺和硫代乙酰胺具有弱诱变性。在所使用的浓度下，己烯雌酚、黄樟素、乙酰胺、尿烷和乙硫氨酸的测试结果为阴性。在浓度高达20mg/ml时，己内酰胺对C658-K42未显示任何诱变作用。叠氮化钠不太可能致癌，但在艾姆斯试验中具有很强的诱变性，对C658-K42显示出非常微弱的诱变作用。
