Nikiforaki Dimitra, Vanden Meerschaut Frauke, de Roo Chloë, Lu Yuechao, Ferrer-Buitrago Minerva, de Sutter Petra, Heindryckx Björn
Ghent Fertility and Stem Cell Team, Department for Reproductive Medicine, Ghent University Hospital, Ghent, Belgium.
Ghent Fertility and Stem Cell Team, Department for Reproductive Medicine, Ghent University Hospital, Ghent, Belgium.
Fertil Steril. 2016 Mar;105(3):798-806.e2. doi: 10.1016/j.fertnstert.2015.11.007. Epub 2015 Nov 26.
To assess the effect of two assisted oocyte activation (AOA) protocols with the use of two calcium (Ca(2+)) ionophores, ionomycin and A23187 (calcimycin), on the intracellular Ca(2+) level in mouse and human oocytes and the fertilization rates.
Comparison of two Ca(2+) ionophores, ionomycin and A23187, regarding their capacity to increase the intracellular Ca(2+) level and to support subsequent oocyte activation and development.
University hospital research laboratory.
PATIENT(S)/ANIMAL(S): Patients undergoing intracytoplasmic sperm injection (ICSI) treatment and B6D2F1 mice.
INTERVENTION(S): Assisted oocyte activation and microinjection of mouse and human oocytes with sperm.
MAIN OUTCOME MEASURE(S): Measurement of the fertilizing and Ca(2+)-releasing ability of human sperm.
RESULT(S): Ionomycin was more potent than A23187 in provoking Ca(2+) increases in both mouse and human oocytes with significantly higher amplitude and area under the receiver operating characteristic curve. The oocyte activation rate was significantly higher when mouse oocytes were activated with the use of the ionomycin- rather than the A23187-based AOA protocol. Furthermore, oocyte activation rate was higher when human in vitro matured oocytes were activated with the ionomycin-based AOA protocol, but the difference did not reach statistical significance.
CONCLUSION(S): In both mouse and human oocytes, the AOA protocol that used ionomycin was more efficient than the one that used A23187. Bearing in mind that mammalian fertilization is successful when the total dose of Ca(2+) released reaches a minimal threshold, the use of ionomycin for human AOA might be justified instead of the use of A23187.
评估使用两种钙（Ca(2+)）离子载体（离子霉素和A23187（钙霉素））的两种辅助卵母细胞激活（AOA）方案对小鼠和人类卵母细胞内Ca(2+)水平及受精率的影响。
比较两种Ca(2+)离子载体（离子霉素和A23187）在提高细胞内Ca(2+)水平以及支持后续卵母细胞激活和发育方面的能力。
大学医院研究实验室。
患者/动物：接受卵胞浆内单精子注射（ICSI）治疗的患者和B6D2F1小鼠。
辅助卵母细胞激活以及对小鼠和人类卵母细胞进行精子显微注射。
测量人类精子的受精和Ca(2+)释放能力。
在引发小鼠和人类卵母细胞Ca(2+)升高方面，离子霉素比A23187更有效，其在受试者操作特征曲线下的幅度和面积显著更高。当使用基于离子霉素而非基于A23187的AOA方案激活小鼠卵母细胞时，卵母细胞激活率显著更高。此外，当使用基于离子霉素的AOA方案激活人类体外成熟卵母细胞时，卵母细胞激活率更高，但差异未达到统计学意义。
在小鼠和人类卵母细胞中，使用离子霉素的AOA方案比使用A23187的方案更有效。鉴于当释放的Ca(2+)总量达到最小阈值时哺乳动物受精才会成功，使用离子霉素进行人类AOA可能比使用A23187更合理。
