Liu Z Z, Xia J H, Xin L L, Wang Z G, Qian L, Wu S G, Yang S L, Li K
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
School of Life Science, Anhui Agricultural University, Anhui Hefei, China.
Genet Mol Res. 2015 Nov 30;14(4):15256-66. doi: 10.4238/2015.November.30.1.
The swine leukocyte antigen (SLA) complex harbors highly polymorphic gene clusters encoding glycoproteins that are involved in responses to vaccines, infectious disease, and production performance. Pigs with well-defined SLA class II genes are useful for the study of disease, immunology, and vaccines. In this study, we analyzed four SLA class II genes (SLA-DRA, SLA-DRB1, SLA-DQA, SLA-DQB1) in 22 founder Guizhou minipigs using a sequence-based typing method. Twelve alleles were detected, compared with the SLA class II allele sequences in the GenBank, and one of twelve alleles was found to be novel in Guizhou minipigs. There are four SLA II haplotypes, and one of them has been previously reported in Meishan pigs. Furthermore, based on sequence information of these alleles, we developed a simple SLA typing method implemented to SLA-typing for unknown offspring of Guizhou minipigs, relying on designed twelve sequence specific primers that could discriminate between each other. According to the combination of sequence-based typing and PCR-SSP, we were able to rapidly check SLA typing of Guizhou breeding stock and identified four SLA haplotypes in the herd. Therefore, SLA-defined Guizhou minipigs will be useful as animal models for xenotransplantation and immunological research.
猪白细胞抗原（SLA）复合体包含高度多态的基因簇，这些基因簇编码参与疫苗反应、传染病反应和生产性能的糖蛋白。具有明确SLA II类基因的猪对于疾病、免疫学和疫苗研究很有用。在本研究中，我们使用基于序列的分型方法分析了22头贵州小型猪种猪的四个SLA II类基因（SLA-DRA、SLA-DRB1、SLA-DQA、SLA-DQB1）。与GenBank中的SLA II类等位基因序列相比，检测到了12个等位基因，其中一个等位基因在贵州小型猪中是新发现的。有四种SLA II单倍型，其中一种先前已在梅山猪中报道过。此外，基于这些等位基因的序列信息，我们开发了一种简单的SLA分型方法，该方法依靠设计的12条序列特异性引物来区分彼此，用于对贵州小型猪未知后代进行SLA分型。根据基于序列的分型和PCR-SSP的组合，我们能够快速检查贵州种猪的SLA分型，并在猪群中鉴定出四种SLA单倍型。因此，明确SLA的贵州小型猪将作为异种移植和免疫学研究的动物模型。
