墨西哥结直肠癌患者的RUNX3基因多态性与单倍型

RUNX3 gene polymorphisms and haplotypes in Mexican patients with colorectal cancer.

作者信息

Suárez-Villanueva S, Ayala-Madrigal M L, Peregrina-Sandoval J, Macías-Gómez N, Ramírez-Ramírez R, Muñiz-Mendoza R, Moreno-Ortiz J M, Centeno-Flores M, Maciel-Gutiérrez V, Cabrales E, Gutiérrez-Angulo M

机构信息

Doctorado en Genética Humana and Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.

Laboratorio de Inmunobiología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco, México.

出版信息

Genet Mol Res. 2015 Dec 1;14(4):15505-10. doi: 10.4238/2015.November.30.28.

DOI:10.4238/2015.November.30.28

PMID:26634516

Abstract

We analyzed a possible association between RUNX3 gene polymorphisms and haplotypes in Mexican patients with colorectal cancer (CRC). Genomic DNA samples were obtained from the peripheral blood of 176 Mexican patients with CRC at diagnosis and from 195 individuals that formed the control group. The polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism. Association was estimated by odds ratio (OR). The haplotypes and linkage disequilibrium were established using the Arlequin v3.5 software. We found that the RUNX3 polymorphisms analyzed were in Hardy-Weinberg equilibrium. The RUNX3 rs2236852 AA genotype and A allele showed association with CRC (OR = 0.39, 95%CI = 0.21-0.73, P < 0.01; OR = 0.65, 95%CI = 0.49-0.87, P < 0.01, respectively), while the rs6672420, rs11249206, and rs760805 polymorphisms did not show significant association with CRC. The TA haplotype (SNPs rs760805 and rs2236852) showed an increased risk for CRC (OR = 2.52, 95%CI = 1.47-4.30, P < 0.001). In conclusion, we found that the AA genotype and A allele of rs2236852 polymorphism confer a decreased CRC risk, while the TA haplotype appears to increase the risk of CRC development in Mexican patients.

摘要

我们分析了墨西哥结直肠癌（CRC）患者中RUNX3基因多态性与单倍型之间的可能关联。基因组DNA样本取自176例确诊的墨西哥CRC患者诊断时的外周血以及195名组成对照组的个体。通过聚合酶链反应-限制性片段长度多态性检测多态性。通过优势比（OR）估计关联性。使用Arlequin v3.5软件确定单倍型和连锁不平衡。我们发现所分析的RUNX3多态性处于哈迪-温伯格平衡。RUNX3 rs2236852 AA基因型和A等位基因与CRC相关（OR = 0.39，95%CI = 0.21 - 0.73，P < 0.01；OR = 0.65，95%CI = 0.49 - 0.87，P < 0.01），而rs6672420、rs11249206和rs760805多态性与CRC无显著关联。TA单倍型（SNP rs760805和rs2236852）显示CRC风险增加（OR = 2.52，95%CI = 1.47 - 4.30，P < 0.001）。总之，我们发现rs2236852多态性的AA基因型和A等位基因使CRC风险降低，而TA单倍型似乎增加了墨西哥患者发生CRC的风险。