Norizadeh Tazehkand Mostafa, Topaktas Mehmet, Yilmaz Mehmet Bertan
a Department of Biotechnology , Institute of Basic and Applied Sciences, Cukurova University , Adana , Turkey .
b Faculty of Science and Letters , Department of Biology, Cukurova University , Adana , Turkey , and.
Drug Chem Toxicol. 2016;39(3):307-11. doi: 10.3109/01480545.2015.1113989. Epub 2015 Dec 4.
4-Methylimidazole (4-MEI) is formed during the production of certain caramel coloring agents used in many food and drink products. It may also be formed during the cooking, roasting, or other processing of some foods and beverages. So it was unintentionally consumed in worldwide. This study was aimed to investigate the genotoxic and cytotoxic effects of 4-MEI using chromosome aberration (CA) and mitotic index (MI) in Swiss Albino mice. In this research, CA and MI of the mouse bone marrow cells were analyzed after treating the animals with 4-MEI (100, 130 and 160 mg/kg) for 12 h and 24 h treatment times. All data were analyzed using statistical methods. 4-MEI significantly increased the percentage of CAs at all concentrations for 12 h and at highest concentration for 24 h treatment periods. 4-MEI at highest concentration for 12 h and at all concentrations for 24 h decreased the MI in comparison with control. Genotoxic and cytotoxic effects of 4-MEI at 24 h treatment periods were concentration dependent. Consequently, it can be said that 4-MEI have genotoxic and cytotoxic effect in mouse.
4-甲基咪唑（4-MEI）在许多食品和饮料产品中使用的某些焦糖色素生产过程中形成。它也可能在一些食品和饮料的烹饪、烘焙或其他加工过程中形成。因此，它在全球范围内被无意摄入。本研究旨在使用瑞士白化小鼠的染色体畸变（CA）和有丝分裂指数（MI）来研究4-MEI的遗传毒性和细胞毒性作用。在本研究中，用4-MEI（100、130和160mg/kg）处理动物12小时和24小时后，分析小鼠骨髓细胞的CA和MI。所有数据均采用统计方法进行分析。在12小时的所有浓度以及24小时处理期的最高浓度下，4-MEI均显著增加了CA的百分比。与对照组相比，12小时最高浓度和24小时所有浓度的4-MEI均降低了MI。24小时处理期的4-MEI的遗传毒性和细胞毒性作用呈浓度依赖性。因此，可以说4-MEI对小鼠具有遗传毒性和细胞毒性作用。
