Mateo-Fernández Marcos, Alves-Martínez Pilar, Del Río-Celestino Mercedes, Font Rafael, Merinas-Amo Tania, Alonso-Moraga Ángeles
Department of Genetic, Rabanales Campus, University of Córdoba (UCO), 14071 Córdoba, Spain.
Agri-Food Laboratory, Council of Agriculture, Fisheries and Rural Development of Andalusia (CAPDER), 14004 Córdoba, Spain.
Foods. 2019 Sep 5;8(9):392. doi: 10.3390/foods8090392.
Nutraceutical activity of food is analysed to promote the healthy characteristics of diet where additives are highly used. Caramel is one of the most worldwide consumed additives and it is produced by heating natural carbohydrates. The aim of this study was to evaluate the food safety and the possible nutraceutical potential of caramel colour class IV (CAR). For this purpose, in vivo toxicity/antitoxicity, genotoxicity/antigenotoxicity and longevity assays were performed using the model. In addition, cytotoxicity, internucleosomal DNA fragmentation, single cell gel electrophoresis and methylation status assays were conducted in the in vitro HL-60 human leukaemia cell line. Our results reported that CAR was neither toxic nor genotoxic and showed antigenotoxic effects in Furthermore, CAR induced cytotoxicity and hipomethylated sat-α repetitive element using HL-60 cell line. In conclusion, the food safety of CAR was demonstrated, since Lethal Dose 50 (LD) was not reached in toxicity assay and any of the tested concentrations induced mutation rates higher than that of the concurrent control in . On the other hand, CAR protected DNA from oxidative stress provided by hydrogen peroxide in . Moreover, CAR showed chemopreventive activity and modified the methylation status of HL-60 cell line. Nevertheless, much more information about the mechanisms of gene therapies related to epigenetic modulation by food is necessary.
分析食品的营养保健活性以促进在大量使用添加剂的情况下饮食的健康特性。焦糖是全球消费最多的添加剂之一,它是通过加热天然碳水化合物生产的。本研究的目的是评估IV类焦糖色素(CAR)的食品安全和可能的营养保健潜力。为此,使用该模型进行了体内毒性/抗毒性、遗传毒性/抗遗传毒性和寿命测定。此外,在体外HL-60人白血病细胞系中进行了细胞毒性、核小体间DNA片段化、单细胞凝胶电泳和甲基化状态测定。我们的结果表明,CAR既无毒性也无遗传毒性,并且在……中显示出抗遗传毒性作用。此外,CAR使用HL-60细胞系诱导细胞毒性并使sat-α重复元件低甲基化。总之,CAR的食品安全得到了证明,因为在毒性试验中未达到半数致死剂量(LD),并且在……中任何测试浓度诱导的突变率均未高于同期对照。另一方面,CAR在……中保护DNA免受过氧化氢提供的氧化应激。此外,CAR显示出化学预防活性并改变了HL-60细胞系的甲基化状态。然而,关于食品表观遗传调控相关基因治疗机制的更多信息是必要的。