Shakeel Muhammad, Rais Afroz, Hassan Muhammad Nadeem, Hafeez Fauzia Yusuf
Department of Biosciences, COMSATS Institute of Information Technology Islamabad, Pakistan.
Front Microbiol. 2015 Nov 18;6:1286. doi: 10.3389/fmicb.2015.01286. eCollection 2015.
Plant associated rhizobacteria prevailing in different agro-ecosystems exhibit multiple traits which could be utilized in various aspect of sustainable agriculture. Two hundred thirty four isolates were obtained from the roots of basmati-385 and basmati super rice varieties growing in clay loam and saline soil at different locations of Punjab (Pakistan). Out of 234 isolates, 27 were able to solubilize zinc (Zn) from different Zn ores like zinc phosphate [Zn3 (PO4)2], zinc carbonate (ZnCO3) and zinc oxide (ZnO). The strain SH-10 with maximum Zn solubilization zone of 24 mm on Zn3 (PO4)2ore and strain SH-17 with maximum Zn solubilization zone of 14-15 mm on ZnO and ZnCO3ores were selected for further studies. These two strains solubilized phosphorous (P) and potassium (K) in vitro with a solubilization zone of 38-46 mm and 47-55 mm respectively. The strains also suppressed economically important rice pathogens Pyricularia oryzae and Fusarium moniliforme by 22-29% and produced various biocontrol determinants in vitro. The strains enhanced Zn translocation toward grains and increased yield of basmati-385 and super basmati rice varieties by 22-49% and 18-47% respectively. The Zn solubilizing strains were identified as Bacillus sp. and Bacillus cereus by 16S rRNA gene analysis.
在不同农业生态系统中普遍存在的植物根际细菌具有多种特性，可用于可持续农业的各个方面。从巴基斯坦旁遮普省不同地点的粘壤土和盐土中种植的巴斯马蒂-385和超级巴斯马蒂水稻品种的根部获得了234株分离物。在234株分离物中，有27株能够从不同的锌矿石中溶解锌（Zn），如磷酸锌[Zn3(PO4)2]、碳酸锌（ZnCO3）和氧化锌（ZnO）。选择在Zn3(PO4)2矿石上锌溶解区最大为24毫米的菌株SH-10和在ZnO和ZnCO3矿石上锌溶解区最大为14 - 15毫米的菌株SH-17进行进一步研究。这两种菌株在体外溶解磷（P）和钾（K），溶解区分别为38 - 46毫米和47 - 55毫米。这些菌株还将经济上重要的水稻病原菌稻瘟病菌和串珠镰刀菌抑制了22 - 29%，并在体外产生了各种生物防治决定因素。这些菌株增强了锌向籽粒的转运，使巴斯马蒂-385和超级巴斯马蒂水稻品种的产量分别提高了22 - 49%和18 - 47%。通过16S rRNA基因分析，将锌溶解菌株鉴定为芽孢杆菌属和蜡样芽孢杆菌。
