Belone Andrea de Faria F, Rosa Patrícia S, Trombone Ana P F, Fachin Luciana R V, Guidella Cássio C, Ura Somei, Barreto Jaison A, Pinilla Mabel G, de Carvalho Alex F, Carraro Dirce M, Soares Fernando A, Soares Cleverson T
Department of Anatomic Pathology, Instituto Lauro de Souza Lima São Paulo, Brazil ; Department of Anatomic Pathology, A.C. Camargo Cancer Center São Paulo, Brazil.
Division of Research and Education, Instituto Lauro de Souza Lima São Paulo, Brazil.
Front Genet. 2015 Nov 20;6:334. doi: 10.3389/fgene.2015.00334. eCollection 2015.
Leprosy, an infectious disease caused by Mycobacterium leprae, affects millions of people worldwide. However, little is known regarding its molecular pathophysiological mechanisms. In this study, a comprehensive assessment of human mRNA was performed on leprosy skin lesions by using DNA chip microarrays, which included the entire spectrum of the disease along with its reactional states. Sixty-six samples from leprotic lesions (10TT, 10BT, 10BB, 10BL, 4LL, 14R1, and 10R2) and nine skin biopsies from healthy individuals were used as controls (CC) (ages ranged from 06 to 83 years, 48 were male and 29 female). The evaluation identified 1580 differentially expressed mRNAs [Fold Change (FC) ≥ 2.0, p ≤ 0.05] in diseased lesions vs. healthy controls. Some of these genes were observed in all forms of the disease (CD2, CD27, chit1, FA2H, FAM26F, GZMB, MMP9, SLAMF7, UBD) and others were exclusive to reactional forms (Type "1" reaction: GPNMB, IL1B, MICAL2, FOXQ1; Type "2" reaction: AKR1B10, FAM180B, FOXQ1, NNMT, NR1D1, PTX3, TNFRSF25). In literature, these mRNAs have been associated with numerous pathophysiological processes and signaling pathways and are present in a large number of diseases. The role of these mRNAs maybe studied in the context of developing new diagnostic markers and therapeutic targets for leprosy.
麻风病是一种由麻风分枝杆菌引起的传染病，影响着全球数百万人。然而，关于其分子病理生理机制却知之甚少。在本研究中，利用DNA芯片微阵列对麻风病皮肤病变进行了人类mRNA的全面评估，该评估涵盖了疾病的整个谱系及其反应状态。来自麻风病病变的66个样本（10个结核样型、10个界线类偏结核样型、10个瘤型、10个界线类偏瘤型、4个麻风样型、14个Ⅰ型反应期和10个Ⅱ型反应期）以及来自健康个体的9个皮肤活检样本用作对照（CC）（年龄范围为6至83岁，48例为男性，29例为女性）。评估发现，与健康对照相比，患病病变中有1580个差异表达的mRNA[倍数变化（FC）≥2.0，p≤0.05]。其中一些基因在疾病的所有形式中均有观察到（CD2、CD27、几丁质酶1、脂肪酸2羟化酶、家族性癌症突变基因26F、颗粒酶B、基质金属蛋白酶9、信号淋巴细胞激活分子家族成员7、泛素结合结构域蛋白），其他一些基因则是反应期所特有的（Ⅰ型反应：糖蛋白非转移性黑色素瘤蛋白B、白细胞介素1β、微管相关蛋白轻链2、叉头框Q1；Ⅱ型反应：醛糖还原酶1B10、家族性癌症突变基因180B、叉头框Q1、烟酰胺N-甲基转移酶、核受体亚家族1D组成员1、五聚体3、肿瘤坏死因子受体超家族成员25）。在文献中，这些mRNA与众多病理生理过程和信号通路相关，且存在于大量疾病中。这些mRNA的作用可在开发麻风病新诊断标志物和治疗靶点的背景下进行研究。
