Sieńko Anna, Wieczorek Piotr, Majewski Piotr, Ojdana Dominika, Wieczorek Anna, Olszańska Dorota, Tryniszewska Elżbieta
Department of Microbiological Diagnostics and Infectious Immunology, Medical University of Bialystok, Bialystok, Poland.
Department of Microbiological Diagnostics and Infectious Immunology, University Hospital of Bialystok, Bialystok, Poland.
Acta Biochim Pol. 2015;62(4):859-66. doi: 10.18388/abp.2015_1147. Epub 2015 Dec 4.
An increase in the antibiotic resistance among Enterococcus faecium strains has been observed worldwide. Moreover, this bacteria has the ability to produce several virulence factors and to form biofilm that plays an important role in human infections. This study was designed to compare the antibiotic resistance and the prevalence of genes encoding surface protein (esp), aggregation substance (as), surface adhesin (efaA), collagen adhesin (ace), gelatinase (gelE), and hialuronidase (hyl) between biofilm-producing and non-producing E. faecium strains. Therefore, ninety E. faecium clinical isolates were tested for biofilm-forming ability, and then were assigned to two groups: biofilm-positive (BIO(+), n =70) and biofilm-negative (BIO(-), n = 20). Comparison of these groups showed that BIO(+) isolates were resistant to β-lactams, whereas 10% of BIO(-) strains were susceptible to ampicillin (statistically significant difference, p = 0.007) and 5% to imipenem. Linezolid and tigecycline were the only antibiotics active against all tested isolates. Analysis of the virulence factors revealed that ace, efaA, and gelE genes occurred more frequently in BIO(-) strains (ace in 50% BIO(+) vs. 75% BIO(-); efaA 44.3% vs. 85%; gelE 2.9% vs. 15%, respectively), while hyl gene appeared more frequently in BIO(+) isolates (87.1% BIO(+) vs. 65% BIO(-)). These differences were significant (p < 0.05). We concluded that BIO(+) strains were more resistant to antibiotics than BIO(-) strains, but interestingly, BIO(-) isolates were characterized by possession of higher virulence capabilities.
在全球范围内，已观察到粪肠球菌菌株的抗生素耐药性有所增加。此外，这种细菌能够产生多种毒力因子并形成生物膜，这在人类感染中起着重要作用。本研究旨在比较产生物膜和不产生物膜的粪肠球菌菌株之间的抗生素耐药性以及编码表面蛋白（esp）、聚集物质（as）、表面黏附素（efaA）、胶原黏附素（ace）、明胶酶（gelE）和透明质酸酶（hyl）的基因的流行情况。因此，对90株粪肠球菌临床分离株进行了生物膜形成能力测试，然后分为两组：生物膜阳性（BIO(+)，n = 70）和生物膜阴性（BIO(-)，n = 20）。这些组的比较表明，BIO(+)分离株对β-内酰胺类抗生素耐药，而10%的BIO(-)菌株对氨苄西林敏感（差异有统计学意义，p = 0.007），5%对亚胺培南敏感。利奈唑胺和替加环素是仅对所有测试分离株有效的抗生素。毒力因子分析显示，ace、efaA和gelE基因在BIO(-)菌株中出现的频率更高（ace在BIO(+)中为50%，在BIO(-)中为75%；efaA分别为44.3%和85%；gelE分别为2.9%和15%），而hyl基因在BIO(+)分离株中出现的频率更高（BIO(+)中为87.1%，BIO(-)中为65%）。这些差异具有统计学意义（p < 0.05）。我们得出结论，BIO(+)菌株比BIO(-)菌株对抗生素更耐药，但有趣的是，BIO(-)分离株具有更高的毒力。
