Jabłońska Joanna, Pawłowski Tomasz, Laskus Tomasz, Zalewska Małgorzata, Inglot Małgorzata, Osowska Sylwia, Perlejewski Karol, Bukowska-Ośko Iwona, Cortes Kamila Caraballo, Pawełczyk Agnieszka, Ząbek Piotr, Radkowski Marek
Department of Hepatology and Acquired Immunodeficiences, Medical University of Warsaw, Warsaw, Poland.
Division of Psychotherapy and Psychosomatic Medicine, Wrocław Medical University, Wrocław, Poland.
BMC Infect Dis. 2015 Dec 4;15:556. doi: 10.1186/s12879-015-1305-1.
Cytokine response against hepatitis C virus (HCV) is likely to determine the natural course of infection as well as the outcome of antiviral treatment. However, the role of particular cytokines remains unclear. The current study analyzed activation of cytokine response in chronic hepatitis C patients undergoing standard antiviral treatment.
Twenty-two patients were treated with pegylated interferon and ribavirin. Twenty-six different cytokine transcripts were measured quantitatively in peripheral blood mononuclear cells (PBMC) before and after therapy and correlated with therapy outcome as well as with clinical and liver histological data.
We found that patients who achieved sustained virological response (SVR) showed higher pretreatment cytokine response when compared to subjects in whom therapy was unsuccessful. The differentially expressed factors included IL-8, IL-16, TNF-α, GM-CSF, MCP-2, TGF-β, and IP-10. Serum ALT activity and/or histological grading also positively correlated with the expression of IL-1α, IL-4, IL-6, IL-10, IL-12, IL-15, GM-CSF, M-CSF, MCP-2 and TGF-β.
Pretreatment activation of the immune system, as reflected by cytokines transcripts upregulation, positively correlates with treatment outcome and closely reflects liver inflammatory activity.
针对丙型肝炎病毒（HCV）的细胞因子反应可能决定感染的自然病程以及抗病毒治疗的结果。然而，特定细胞因子的作用仍不清楚。本研究分析了接受标准抗病毒治疗的慢性丙型肝炎患者细胞因子反应的激活情况。
22例患者接受聚乙二醇干扰素和利巴韦林治疗。在治疗前后对外周血单个核细胞（PBMC）中的26种不同细胞因子转录本进行定量测量，并将其与治疗结果以及临床和肝脏组织学数据相关联。
我们发现，与治疗未成功的受试者相比，实现持续病毒学应答（SVR）的患者在治疗前表现出更高的细胞因子反应。差异表达的因子包括IL-8、IL-16、TNF-α、GM-CSF、MCP-2、TGF-β和IP-10。血清ALT活性和/或组织学分级也与IL-1α、IL-4、IL-6、IL-10、IL-12、IL-15、GM-CSF、M-CSF、MCP-2和TGF-β的表达呈正相关。
细胞因子转录本上调所反映的治疗前免疫系统激活与治疗结果呈正相关，并密切反映肝脏炎症活动。
