Yida Zhang, Imam Mustapha Umar, Ismail Maznah, Wong WaiTeng, Abdullah Maizaton Atmadini, Ideris Aini, Ismail Norsharina
Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia.
Cardiology Department, Affiliated Hospital of Chengde Medical University, Chengde, China.
Food Nutr Res. 2015 Dec 4;59:29046. doi: 10.3402/fnr.v59.29046. eCollection 2015.
N-Acetylneuraminic acid (Neu5Ac), a type of sialic acid, has close links with cholesterol metabolism and is often used as a biomarker in evaluating the risk of cardiovascular diseases. However, most studies on the health implications of Neu5Ac have focused on its effects on the nervous system, while its effects on cardiovascular risk factors have largely been unreported. Thus, the effects of Neu5Ac on coagulation status in high fat diet (HFD)-induced hyperlipidemic rats were evaluated in this study.
Sprague Dawley male rats were divided into five different groups and fed with HFD alone, HFD low-dose Neu5Ac, HFD high-dose Neu5Ac, HFD simvastatin (10 mg/kg day), and normal pellet alone. Food was given ad libitum while body weight of rats was measured weekly. After 12 weeks of intervention, rats were sacrificed and serum and tissue samples were collected for biochemistry and gene expression analysis, respectively.
The results showed that Neu5Ac could improve lipid metabolism and hyperlipidemia-associated coagulation. Neu5Ac exerted comparable or sometimes better physiological effects than simvastatin, at biochemical and gene expression levels.
The data indicated that Neu5Ac prevented HFD-induced hyperlipidemia and associated hypercoagulation in rats through regulation of lipid-related and coagulation-related genes and, by extension, induced metabolite and protein changes. The implications of the present findings are that Neu5Ac may be used to prevent coagulation-related cardiovascular events in hyperlipidemic conditions. These findings are worth studying further.
N-乙酰神经氨酸（Neu5Ac）作为一种唾液酸，与胆固醇代谢密切相关，常被用作评估心血管疾病风险的生物标志物。然而，大多数关于Neu5Ac对健康影响的研究都集中在其对神经系统的作用上，而其对心血管危险因素的影响在很大程度上尚未见报道。因此，本研究评估了Neu5Ac对高脂饮食（HFD）诱导的高脂血症大鼠凝血状态的影响。
将雄性Sprague Dawley大鼠分为五组，分别给予单纯高脂饮食、高脂饮食低剂量Neu5Ac、高脂饮食高剂量Neu5Ac、高脂饮食辛伐他汀（10 mg/kg/天）以及单纯正常颗粒饲料。自由采食，每周测量大鼠体重。干预12周后，处死大鼠，分别收集血清和组织样本进行生化和基因表达分析。
结果表明，Neu5Ac可改善脂质代谢和高脂血症相关的凝血功能。在生化和基因表达水平上，Neu5Ac发挥了与辛伐他汀相当甚至有时更好的生理作用。
数据表明，Neu5Ac通过调节脂质相关和凝血相关基因，进而诱导代谢物和蛋白质变化，预防了HFD诱导的大鼠高脂血症和相关的高凝状态。本研究结果的意义在于，Neu5Ac可能用于预防高脂血症情况下与凝血相关的心血管事件。这些发现值得进一步研究。
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