Guo Yong, Wang Yang, Liu Yinqin, Wang Haitao, Guo Chun, Zhang Xizheng
College of Biotechnology, Guilin Medical University, Guilin 541004, China.
Chin J Traumatol. 2015;18(3):150-6. doi: 10.1016/j.cjtee.2014.09.004.
To investigate the influence of the same mechanical loading on osteogenesis and osteoclastogenesis in vitro.
Primary osteoblasts, bone marrow-derived mesenchymal stem cells (BMSCs, cultured in osteoinductive medium) and RAW264.7 cells cultured in osteoclast inductive medium were all subjected to a 1000 μstrain (μs) at 1 Hz cyclic mechanical stretch for 30 min (twice a day).
After mechanical stimulation, the alkaline phosphatase (ALP) activity, osteocalcin protein level of the osteoblasts and BMSCs were all enhanced, and the mRNA levels of ALP and collagen type I increased. Additionally, extracellular-deposited calcium of both osteoblasts and BMSCs increased. At the same time, the activity of secreted tartrate-resistant acid phosphatase, the number of tartrate-resistant acid phosphatase-positive multinucleated cells, matrix metalloproteinase-9 protein levels of RAW264.7 cells and the extracellular calcium solvency all decreased.
The results demonstrated that 1000 μs cyclic mechanical loading enhanced osteoblasts activity, promoted osteoblastic differentiation of BMSCs and restrained osteoclastogenesis of RAW264.7 cells in vitro.
研究相同机械负荷对体外成骨和破骨细胞生成的影响。
将原代成骨细胞、在成骨诱导培养基中培养的骨髓间充质干细胞（BMSCs）以及在破骨细胞诱导培养基中培养的RAW264.7细胞均置于1000微应变（μs）、1赫兹的循环机械拉伸下30分钟（每天两次）。
机械刺激后，成骨细胞和BMSCs的碱性磷酸酶（ALP）活性、骨钙素蛋白水平均增强，且ALP和I型胶原的mRNA水平升高。此外，成骨细胞和BMSCs的细胞外沉积钙均增加。同时，RAW264.7细胞的抗酒石酸酸性磷酸酶活性、抗酒石酸酸性磷酸酶阳性多核细胞数量、基质金属蛋白酶-9蛋白水平以及细胞外钙溶解性均降低。
结果表明，1000μs循环机械负荷可增强体外成骨细胞活性，促进BMSCs向成骨细胞分化，并抑制RAW264.7细胞的破骨细胞生成。
